T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells.
T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells.
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葡萄球菌肠毒素刺激T细胞刺激。克隆的可变响应和对附件或靶细胞上的主要组织相容性复合物II类分子的需求。
DOI：
10.1084/jem.167.5.1697
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发表时间：
1988-05-01
影响因子：
15.3
通讯作者：
SCHREZENMEIER, H
中科院分区：
文献类型：
--
作者：
FLEISCHER, B;SCHREZENMEIER, H
葡萄球菌肠毒素（SE）是已知的T淋巴细胞最有效的丝裂原；小于10(-9)M的浓度足以激活T细胞。SE激活T细胞的机制尚不清楚。我们利用克隆的人细胞毒性T淋巴细胞和增殖T淋巴细胞来剖析SE活化T细胞的分子机制。除少数例外，所有CD4+或CD8+表型的TCR α / β链表达的T细胞克隆，以及CD4-8- TCR α / β链负链表达的T淋巴细胞克隆，对SE有增殖和/或细胞毒性反应。为了触发所有这些克隆，必须在辅助细胞或靶细胞上存在自体或异体MHC II类分子。这种对II类抗原的需求不是由于对加工过的SE的免疫识别，因为抑制抗原加工对T细胞对SE的反应没有影响。SE直接作用于T细胞，因为(a)它们刺激T细胞系或纯化T细胞的细胞内钙浓度升高，(b)在SE对静止T细胞的增殖激活中，辅助细胞可以被磷酸酯取代。此外，T细胞对SE的反应表现出广泛的克隆异质性。这些结果表明SE是功能上的二价丝裂原，高度选择性地结合HLA II类分子和TCR。因此，与其他多克隆T细胞激活剂相比，SE的激活最接近于T淋巴细胞MHC限制性抗原识别的生理方式。
Staphylococcal enterotoxins (SE) are the most potent mitogens for T lymphocytes known; concentrations of less than 10(-9) M are sufficient for T cell activation. The mechanism of T cell activation by SE is unknown. We have used cloned human cytotoxic and proliferative T lymphocytes to dissect the molecular mechanism of T cell activation by SE. With rare exceptions, all TCR alpha/beta chain-expressing T cell clones of CD4+ or CD8+ phenotype, as well as CD4-8- TCR alpha/beta chain negative chain-expressing T lymphocyte clones, respond with proliferation and/or cytotoxicity to SE. For triggering of all these clones, the presence of autologous or allogeneic MHC class II molecules on accessory or target cells is necessary. This requirement for class II antigens is not due to an immunological recognition of processed SE, since inhibition of antigen processing has no influence on the T cell response to SE. SE acts on the T cells directly since (a) they stimulate a rise in intracellular calcium concentration in T cell lines or purified T cells, and (b) accessory cells can be replaced by phorbolesters in the proliferative activation of resting T cells by SE. Furthermore, the T cell response to SE shows extensive clonal heterogeneity. These results suggest that SE are functionally bivalent mitogens binding highly selectively to HLA class II molecules and the TCR. Thus, compared with other polyclonal T cell activating agents, activation with SE most closely mimicks the physiological way of MHC- restricted antigen recognition by T lymphocytes.
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影响因子：
5.4
作者：
FLEISCHER, B
通讯作者：
FLEISCHER, B
影响因子：
5.4
作者：
LYNCH, DH;COLE, BC;HODES, RJ
通讯作者：
HODES, RJ
影响因子：
5.4
作者：
KIMURA, A;ERSSON, B
通讯作者：
ERSSON, B
影响因子：
64.8
作者：
BANK, I;DEPINHO, RA;CHESS, L
通讯作者：
CHESS, L
影响因子：
5.4
作者：
FLEISCHER, B
通讯作者：
FLEISCHER, B