A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity
A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity
复制标题
两种不同紫外敏感度的水稻品种叶片对增强型 UV-B 辐射响应的蛋白质组学分析
DOI：
10.1007/s12374-011-9162-y
复制
发表时间：
2011-04
影响因子：
2.9
通讯作者：
Zhong-Liang Huang
中科院分区：
文献类型：
--
作者：
Xing-Chun Wu;Chang-Xun Fang;Jin-Yang Chen;Qing-Shui Wang;Ting Chen;Wen-Xiong Lin;Zhong-Liang Huang
为了确定紫外辐射对两个品种Lemont（耐紫外）和dulal（紫外敏感）的蛋白质组学响应，将两个品种分别暴露在自然和增强紫外- b （UV- b）照射下1、7和14 d，并采用二维凝胶电泳、质谱（MS）和生物信息学相结合的方法比较了两个品种叶片蛋白质组学响应的差异。39种蛋白在UV-B处理后上调或下调。其中，有30种的丰度增加或减少了1.5倍以上。进一步采用基质辅助激光解吸/电离飞行时间谱进行了测试，并进行了数据库检索。这样就鉴定出了24种蛋白质。这些鉴定的蛋白在Lemont中大部分上调，而在dulal中只有14个上调。与糖代谢和脂肪酸代谢、信号转导、蛋白合成和折叠有关的9个蛋白在dulal中没有变化。这些结果表明，UV-B对水稻叶片蛋白质组的影响存在复杂的调控机制。
To determine the proteomic response to UV irradiation, two cultivars, i.e., Lemont (UV tolerant) and Dular (UV sensitive), were exposed to natural and enhanced ultraviolet-B (UV-B) irradiation for 1, 7, and 14 days, and two-dimensional gel electrophoresis in combination with mass spectrometry (MS) and bioinformatics were used to compare the different proteomic responses in the leaves of the two cultivars. Thirty-nine proteins were up- or downregulated following the UV-B treatments. Among them, 30 increased or decreased more than 1.5-fold in abundance. They were further tested by using matrix-assisted laser desorption/ionization time of flight MS and performed a database search. Twenty-four proteins were thus identified. These identified proteins were mostly upregulated in Lemont, whereas only 14 of them upregulated in Dular. Nine proteins involved in glycometabolism and fatty acid metabolisms, signal transduction, and protein synthesis and folding in Dular were not changed. These results suggest that there was a complex regulative mechanism on the proteomes in rice leaves upon UV-B exposure.
登录
查看更多内容
DOI：
10.1071/pp9940563
发表时间：
1994
期刊：
Australian Journal of Plant Physiology
影响因子：
--
作者：
J. He;L.-K. Huang;W. Chow;M. Whitecross;JM Anderson
通讯作者：
J. He;L.-K. Huang;W. Chow;M. Whitecross;JM Anderson
影响因子：
5.4
作者：
Tadashi Sato;T. Ueda;Y. Fukuta;Tadashi Kumagai;Masahiro Yano
通讯作者：
Tadashi Sato;T. Ueda;Y. Fukuta;Tadashi Kumagai;Masahiro Yano
DOI：
10.1016/j.bbrc.2008.12.067
发表时间：
2009-01-30
影响因子：
3.1
作者：
Ke, Yuqin;Han, Guoqiang;Li, Jiaxu
通讯作者：
Li, Jiaxu
影响因子：
5.2
作者：
Goto, M;Ehara, H;Morita, O
通讯作者：
Morita, O
葡萄藤（Vitis Vinifera L.）CV的转录组变化。由紫外线辐射引起的马尔贝克叶。
影响因子：
5.3
作者：
Pontin MA;Piccoli PN;Francisco R;Bottini R;Martinez-Zapater JM;Lijavetzky D
通讯作者：
Lijavetzky D