Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation.
Transcriptome changes in grapevine (Vitis vinifera L.) cv. Malbec leaves induced by ultraviolet-B radiation.
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葡萄藤（Vitis Vinifera L.）CV的转录组变化。由紫外线辐射引起的马尔贝克叶。
DOI：
10.1186/1471-2229-10-224
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发表时间：
2010-10-20
影响因子：
5.3
通讯作者：
Lijavetzky D
中科院分区：
文献类型：
--
作者：
Pontin MA;Piccoli PN;Francisco R;Bottini R;Martinez-Zapater JM;Lijavetzky D
紫外线-B辐射（UV-B，280-315 nm）是太阳光的一种天然成分，对植物的生理具有多种调节作用。对UV-B的反应的性质取决于UV-B处理的注量率、剂量、持续时间和波长。有报道利用基因芯片技术分析了UV-B光对几种植物基因表达的影响。然而，没有关于葡萄藤中UV-B引发的转录组响应的信息。本文研究了离体培养的葡萄属葡萄品种（Vitis vinifera cv.）使用新定制的GrapeGen Affytron GeneChip®，以两种不同的注量率（16 h，18.25 μW cm-2，4 h，133 μW cm-2）对Malbec植物进行相同剂量的生物有效UV-B辐射（4.75 kJ m-2 d-1）。受高通量率UV-B调控的基因数量是受低通量UV-B调控的基因数量的两倍。他们的功能分析揭示了几个功能类别通常由UV-B治疗以及类别更具体地调制取决于UV-B注量率。一般的保护反应，即诱导的途径调节UV-B吸收化合物的合成，如苯丙烷途径，诱导不同的抗氧化防御系统和激活的途径通常与病原体防御和非生物胁迫反应似乎发挥关键作用，葡萄藤对UV-B辐射的反应。此外，高通量率UV-B似乎专门调制额外的途径和过程，以保护葡萄藤幼苗免受UV-B诱导的氧化胁迫，停止细胞周期的进展，并控制蛋白质降解。另一方面，低通量率UV-B调节生长素和脱落酸的代谢，以及在细胞壁的修改，可能参与UV-B的驯化过程中的具体反应的表达。结果表明，UV-B辐射对葡萄藤（葡萄属vinifera cv. Malbec）小植株。功能类别通常调制下的UV-B的治疗以及转录具体调控的UV-B强度依赖的方式进行了鉴定。高通量率UV-B主要对防御或一般的多重胁迫反应途径具有调节作用，而低通量率UV-B促进了可能参与UV-B保护或减轻UV-B诱导的损伤的基因的表达。这项研究还提供了一个广泛的基因清单，调节葡萄藤对UV-B的反应中涉及的多种代谢途径，可用于未来的研究。
Ultraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology. However, there is no information on the transcriptome response triggered by UV-B in grapevine. In this paper we investigate the gene expression responses of leaves from in vitro cultured Vitis vinifera cv. Malbec plants subjected to the same dose of biologically effective UV-B radiation (4.75 kJ m-2 d-1) administered at two different fluence rates (16 h at ≅ 8.25 μW cm-2, 4 h at ≅ 33 μW cm-2) using a new custom made GrapeGen Affymetrix GeneChip®. The number of genes modulated by high fluence rate UV-B doubled the number of genes modulated by low fluence UV-B. Their functional analyses revealed several functional categories commonly regulated by both UV-B treatments as well as categories more specifically modulated depending on UV-B fluence rate. General protective responses, namely the induction of pathways regulating synthesis of UV-B absorbing compounds such as the Phenylpropanoid pathway, the induction of different antioxidant defense systems and the activation of pathways commonly associated with pathogen defense and abiotic stress responses seem to play critical roles in grapevine responses against UV-B radiation. Furthermore, high fluence rate UV-B seemed to specifically modulate additional pathways and processes in order to protect grapevine plantlets against UV-B-induced oxidative stress, stop the cell cycle progression, and control protein degradation. On the other hand, low fluence rate UV-B regulated the expression of specific responses in the metabolism of auxin and abscisic acid as well as in the modification of cell walls that could be involved in UV-B acclimation-like processes. Our results show the UV-B radiation effects on the leaf transcriptome of grapevine (Vitis vinifera cv. Malbec) plantlets. Functional categories commonly modulated under both UV-B treatments as well as transcripts specifically regulated in an UV-B-intensity dependent way were identified. While high fluence rate UV-B had regulatory effects mainly on defense or general multiple-stress responses pathways, low fluence rate UV-B promoted the expression of genes that could be involved in UV-B protection or the amelioration of the UV-B-induced damage. This study also provides an extensive list of genes regulating multiple metabolic pathways involved in the response of grapevine to UV-B that can be used for future researches.
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影响因子：
12.3
作者：
Casati P;Walbot V
通讯作者：
Walbot V
影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
11.4
作者：
de Torres-Zabala, Marta;Truman, William;Grant, Murray
通讯作者：
Grant, Murray
DOI：
10.1016/s1011-1344(01)00152-x
发表时间：
2001-09-01
影响因子：
5.4
作者：
Ballaré, CL;Rousseaux, MC;Scopel, AL
通讯作者：
Scopel, AL
影响因子：
7.3
作者：
Berli, Federico J.;Moreno, Daniela;Bottini, Ruben
通讯作者：
Bottini, Ruben