Deciphering Antibody Responses to Orthonairoviruses in Ruminants.
Deciphering Antibody Responses to Orthonairoviruses in Ruminants.
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破译反刍动物对正病毒的抗体反应。
DOI：
10.3390/microorganisms9071493
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发表时间：
2021-07-13
期刊：
影响因子：
4.5
通讯作者：
Groschup MH
中科院分区：
文献类型：
--
作者：
Hartlaub J;Keller M;Groschup MH
相关病毒之间的抗体交叉反应是常见的诊断挑战，导致诊断特异性和敏感性降低。在这项研究中，研究了被忽视的原鼻空气病毒属成员——哈扎拉病毒（HAZV）、杜格病毒（DUGV）和内罗毕羊病原鼻空气病毒（NSDV）之间的抗体交叉反应。采用同源和异源病毒特异性检测方法，即基于重组N蛋白的间接elisa法、间接免疫荧光法（iIFA）和两种中和试验形式(斑块减少中和试验（PRNT）和微病毒中和试验（mVNT），对实验性感染后的单特异性羊和牛血清以及HAZV、DUGV和NSDV免疫试验进行检测。elisa的特异性最高，其次是mVNT、iIFA和PRNT。交叉反应主要在内罗毕羊病血清群中观察到，但令人惊讶的是，PRNT中的HAZV抗体也能中和NSDV和DUGV。总之，我们推荐elisa和mvnt作为鉴别诊断方法来区分这些抗体。NSDV抗血清也用于检测人病原体克里米亚-刚果出血热病毒（CCHFV）抗体的血清学检测。有趣的是，所有CCHFV elisa（内部和商业）都具有很高的诊断特异性，而在CCHFV iIFA中观察到显著的交叉反应。以前，在分析HAZV和DUGV抗血清时获得了类似的结果。
Antibody cross-reactivities between related viruses are common diagnostic challenges, resulting in reduced diagnostic specificities and sensitivities. In this study, antibody cross-reactions between neglected members of the genus Orthonairovirus—Hazara (HAZV), Dugbe (DUGV), and Nairobi sheep disease orthonairovirus (NSDV)—were investigated. Mono-specific ovine and bovine sera following experimental infections as well immunization trials with HAZV, DUGV, and NSDV were tested in homologous and heterologous virus-specific assays, namely indirect ELISAs based on recombinant N protein, indirect immunofluorescence assays (iIFA), and two neutralization test formats (plaque reduction neutralization test (PRNT) and micro-virus neutralization test (mVNT)). The highest specificities were achieved with the ELISAs, followed by the mVNT, iIFA, and PRNT. Cross-reactivities were mainly observed within the Nairobi sheep disease serogroup–but surprisingly, HAZV antibodies in PRNT did also neutralize NSDV and DUGV. In conclusion, we recommend ELISAs and mVNTs for a discriminative diagnostic approach to differentiate between these antibodies. NSDV antisera were also used in serological assays for the detection of antibodies against the human pathogen Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV). Interestingly, all CCHFV ELISAs (In-house and commercial) achieved high diagnostic specificities, whereas significant cross-reactivities were observed in a CCHFV iIFA. Previously, similar results were obtained when analyzing the HAZV and DUGV antisera.
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DOI：
10.3390/v13030372
发表时间：
2021-02-26
期刊：
Viruses
影响因子：
--
作者：
Hartlaub J;von Arnim F;Fast C;Mirazimi A;Keller M;Groschup MH
通讯作者：
Groschup MH
影响因子：
4.5
作者：
Hartlaub J;von Arnim F;Fast C;Somova M;Mirazimi A;Groschup MH;Keller M
通讯作者：
Keller M
影响因子：
3.8
作者：
Mertens, Marc;Vatansever, Zati;Groschup, Martin H.
通讯作者：
Groschup, Martin H.
DOI：
10.1016/s0035-9203(96)90468-x
发表时间：
1996-01-01
影响因子：
2.2
作者：
Guilherme, JM;GonellaLegall, C;Vincent, J
通讯作者：
Vincent, J
影响因子：
2.7
作者：
Lasecka L;Baron MD
通讯作者：
Baron MD