Rapid Virucidal Activity of Japanese Saxifraga Species-Derived Condensed Tannins against SARS-CoV-2, Influenza A Virus, and Human Norovirus Surrogate Viruses.
Rapid Virucidal Activity of Japanese Saxifraga Species-Derived Condensed Tannins against SARS-CoV-2, Influenza A Virus, and Human Norovirus Surrogate Viruses.
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DOI：
10.1128/aem.00237-23
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发表时间：
2023-06-28
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
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作者：
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)、甲型流感病毒(IAV)和诺如病毒是威胁人类健康的全球性病毒。有效的杀病毒剂的应用有助于灭活手和环境表面的病毒，这对促进强有力的病毒感染控制措施非常重要。天然来源的杀毒消毒剂因其安全和生态友好的特性而引起人们的关注。在这项研究中，我们发现日本虎耳草的多种物种衍生部分对SARS-CoV-2祖先株和多个变异株IAV以及两种人类诺如病毒替代品：猫杯状病毒(FCV)和小鼠诺如病毒(MNV)显示出快速、有效的杀毒活性。缩合单宁被确定为活性化学成分，在这些组分的抗病毒活性中发挥核心作用。在浓度为25 μg/mL时，纯化的浓缩单宁组分SST-2R在10 S作用时间内可显著降低SARS-CoV-2祖系毒株的病毒滴度(分别减少3.13、3.00和2.50log1050%组织培养感染量[≥_(50)]/mL)。当SST-2R浓度为100 μg/mL时，1 m in内病毒滴度下降1.75log10TCID50/m L。Western blotting和透射电子显微镜分析表明，SST-2R导致病毒结构蛋白和包膜结构异常，导致病毒颗粒被破坏。此外，虎耳草植物衍生组分乳膏在10 min内对多种病毒具有杀灭活性。我们的发现表明，虎耳草物种衍生的含有缩合单宁的部分可以用作消毒剂，以对抗手部和环境表面的多种病毒。重要性SARS-CoV-2、IAV和诺如病毒都是高度传染性的病原体。使用天然衍生成分作为新的抗病毒/抗病毒药物目前引起了人们的注意。我们发现，虎耳草属植物提取物的溶液和乳膏形式，对SARS-CoV-2、IAV和人类诺如病毒的替代品具有强大的、快速的杀灭病毒活性。缩合单宁被发现在这一活动中起着核心作用。在具有一定杀灭病毒活性的浓度下，纯化的缩合单宁部分的体外细胞毒性低于常用的杀灭病毒消毒剂70%乙醇或2,000 ppm次氯酸钠溶液。我们的研究表明，含有缩合单宁的虎耳草物种衍生部分可以作为安全的杀病毒剂用于手和环境表面，对抗多种病毒。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), and norovirus are global threats to human health. The application of effective virucidal agents, which contribute to the inactivation of viruses on hands and environmental surfaces, is important to facilitate robust virus infection control measures. Naturally derived virucidal disinfectants have attracted attention owing to their safety and eco-friendly properties. In this study, we showed that multiple Japanese Saxifraga species-derived fractions demonstrated rapid, potent virucidal activity against the SARS-CoV-2 ancestral strain and multiple variant strains, IAV, and two human norovirus surrogates: feline calicivirus (FCV) and murine norovirus (MNV). Condensed tannins were identified as active chemical constituents that play a central role in the virucidal activities of these fractions. At a concentration of 25 μg/mL, the purified condensed tannin fraction Sst-2R induced significant reductions in the viral titers of the SARS-CoV-2 ancestral strain, IAV, and FCV (reductions of ≥3.13, ≥3.00, and 2.50 log10 50% tissue culture infective doses [TCID50]/mL, respectively) within 10 s of reaction time. Furthermore, at a concentration of 100 μg/mL, Sst-2R induced a reduction of 1.75 log10 TCID50/mL in the viral titers of MNV within 1 min. Western blotting and transmission electron microscopy analyses revealed that Sst-2R produced structural abnormalities in viral structural proteins and envelopes, resulting in the destruction of viral particles. Furthermore, Saxifraga species-derived fraction-containing cream showed virucidal activity against multiple viruses within 10 min. Our findings indicate that Saxifraga species-derived fractions containing condensed tannins can be used as disinfectants against multiple viruses on hands and environmental surfaces. IMPORTANCE SARS-CoV-2, IAV, and norovirus are highly contagious pathogens. The use of naturally derived components as novel virucidal/antiviral agents is currently attracting attention. We showed that fractions from extracts of Saxifraga species, in the form of a solution as well as a cream, exerted potent, rapid virucidal activities against SARS-CoV-2, IAV, and surrogates of human norovirus. Condensed tannins were found to play a central role in this activity. The in vitro cytotoxicity of the purified condensed tannin fraction at a concentration that exhibited some extent of virucidal activity was lower than that of 70% ethanol or 2,000 ppm sodium hypochlorite solution, which are popular virucidal disinfectants. Our study suggests that Saxifraga species-derived fractions containing condensed tannins can be used on hands and environmental surfaces as safe virucidal agents against multiple viruses.
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DOI：
10.3390/v13122399
发表时间：
2021-11-30
期刊：
Viruses
影响因子：
--
作者：
Lucero Y;Matson DO;Ashkenazi S;George S;O’Ryan M
通讯作者：
O’Ryan M
影响因子：
4.6
作者：
Sato S;Matsumoto N;Hisaie K;Uematsu S
通讯作者：
Uematsu S
影响因子：
3.4
作者：
Borisova, Marina P.;Kataev, Anatoly A.;Sivozhelezov, Victor S.
通讯作者：
Sivozhelezov, Victor S.
影响因子：
7.6
作者：
NAKAYAMA, M;SUZUKI, K;SHIMAMURA, T
通讯作者：
SHIMAMURA, T
影响因子：
3
作者：
Soltis, DE;Kuzoff, RK;Ferguson, K
通讯作者：
Ferguson, K