Activated CD8 T cells redistribute to antigen-free lymph nodes and exhibit effector and memory characteristics.
Activated CD8 T cells redistribute to antigen-free lymph nodes and exhibit effector and memory characteristics.
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DOI：
10.4049/jimmunol.181.3.1814
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发表时间：
2008-08-01
期刊：
影响因子：
--
通讯作者：
Engelhard VH
中科院分区：
文献类型：
--
作者：
Brinkman CC;Sheasley-O'Neill SL;Ferguson AR;Engelhard VH
“这是一个作者制作的版本的手稿接受发表在免疫学杂志（JI）。美国免疫学家协会(AAI)JI的出版商拥有本手稿的版权。这一版本的手稿尚未由联合执行进行复制编辑或编辑校对;因此，它可能与联合执行发表的最后版本（在线和印刷版）不同。AAI（JI）对作者制作的手稿版本或美国国立卫生研究院或任何其他第三方从中衍生的任何版本中的错误或遗漏不承担责任。记录的最后可引用版本可在www.jimmunol.org上找到。”外源性树突状细胞在体内注射时显示出有限的运输，并刺激定位于少量淋巴区室的CD 8 T细胞应答。通过检查在存在和不存在FTY 720（一种导致淋巴结中T细胞隔离的药物）的情况下的这些应答，我们证明了在活化的3天内，分裂的CD 8 T细胞的显著部分重新分布到无抗原的淋巴结中。尽管CD 62 L的表达水平存在差异，但这些细胞的再分布是CD 62 L依赖性的。重分布的CD 8 T细胞表现出分化效应子的特征。然而，当在活化后3天从无抗原淋巴结中重新分离并转移到幼稚小鼠中时，它们持续至少3周并在抗原激发后扩增。因此，免疫后3天重新分布到无抗原淋巴结的CD 8 T细胞含有记忆前体。我们认为，这种再分配过程代表了一个重要的机制，建立淋巴结驻留中央记忆，再分配到无抗原的节点是一个额外的特征，以区分记忆的前体从终端效应。
“This is an author-produced version of a manuscript accepted for publication in The Journal of Immunology (The JI). The American Association of Immunologists, Inc. (AAI), publisher of The JI, holds the copyright to this manuscript. This version of the manuscript has not yet been copyedited or subjected to editorial proofreading by The JI; hence, it may differ from the final version published in The JI (online and in print). AAI (The JI) is not liable for errors or omissions in this author-produced version of the manuscript or in any version derived from it by the U.S. National Institutes of Health or any other third party. The final, citable version of record can be found at www.jimmunol.org.” Exogenous dendritic cells display restricted trafficking when injected in vivo and stimulate CD8 T cell responses that are localized to a small number of lymphoid compartments. By examining these responses in the presence and absence of FTY720, a drug that causes sequestration of T cells in lymph nodes, we demonstrate that a significant fraction of divided CD8 T cells redistribute into antigen-free lymph nodes within 3 days of activation. Despite variation in the level of expression of CD62L, redistribution of these cells is CD62L-dependent. Redistributed CD8 T cells exhibit characteristics of differentiated effectors. However, when re-isolated from antigen-free lymph nodes 3 days after activation and transferred into naïve mice, they persist for at least 3 weeks and expand upon antigen challenge. Thus, CD8 T cells that redistribute to antigen-free lymph nodes 3 days after immunization contain memory precursors. We suggest that this redistribution process represents an important mechanism for establishment of lymph node resident central memory, and that redistribution to antigen-free nodes is an additional characteristic to be added to those that distinguish memory precursors from terminal effectors.
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影响因子：
64.8
作者：
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通讯作者：
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作者：
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5.4
作者：
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