Genome-Wide Association Study and QTL Mapping Reveal Genomic Loci Associated with Fusarium Ear Rot Resistance in Tropical Maize Germplasm.
Genome-Wide Association Study and QTL Mapping Reveal Genomic Loci Associated with Fusarium Ear Rot Resistance in Tropical Maize Germplasm.
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DOI：
10.1534/g3.116.034561
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发表时间：
2016-12-07
期刊：
影响因子：
--
通讯作者：
Mahuku G
中科院分区：
文献类型：
--
作者：
Chen J;Shrestha R;Ding J;Zheng H;Mu C;Wu J;Mahuku G
由轮枝镰刀菌引起的镰孢穗腐病（FER）是玉米的一种主要病害，在全球范围内降低了谷物品质。宿主抗性是控制疾病的最合适的策略。我们报告了全基因组关联研究（GWAS）的结果，以检测一组818个热带玉米自交系在三个环境中评估的FER抗性增加相关的等位基因。使用43，424个单核苷酸多态性（SNP）标记进行的关联测试确定了45个SNP和15个单倍型与FER抗性显著相关。每个相关的SNP位点对抗病性的加性效应相对较小，占性状变异的1-4%。这些SNP和单倍型位于38个候选基因内或邻近，其中21个是与植物对胁迫的耐受性相关的候选基因，包括抗病性。在4个双亲群体中进行连锁定位，以验证GWAS结果，确定了15个与F。轮枝病抗性将GWAS和QTL整合到玉米物理图谱上显示了位于第2、3、4、5、9和10号染色体上的8个共位位点。第2和第9染色体上的QTL是新的。这些结果表明，FER抗性是一个复杂的性状，是由多个基因的影响较小。在鉴定的标记上选择用于提高FER抗性的价值是有限的;相反，选择联合收割机小效应抗性等位基因结合针对目标和一般适应性状的多基因背景的基因组选择可能对于提高玉米的FER抗性是富有成效的。
Fusarium ear rot (FER) incited by Fusarium verticillioides is a major disease of maize that reduces grain quality globally. Host resistance is the most suitable strategy for managing the disease. We report the results of genome-wide association study (GWAS) to detect alleles associated with increased resistance to FER in a set of 818 tropical maize inbred lines evaluated in three environments. Association tests performed using 43,424 single-nucleotide polymorphic (SNPs) markers identified 45 SNPs and 15 haplotypes that were significantly associated with FER resistance. Each associated SNP locus had relatively small additive effects on disease resistance and accounted for 1–4% of trait variation. These SNPs and haplotypes were located within or adjacent to 38 candidate genes, 21 of which were candidate genes associated with plant tolerance to stresses, including disease resistance. Linkage mapping in four biparental populations to validate GWAS results identified 15 quantitative trait loci (QTL) associated with F. verticillioides resistance. Integration of GWAS and QTL to the maize physical map showed eight colocated loci on chromosomes 2, 3, 4, 5, 9, and 10. QTL on chromosomes 2 and 9 are new. These results reveal that FER resistance is a complex trait that is conditioned by multiple genes with minor effects. The value of selection on identified markers for improving FER resistance is limited; rather, selection to combine small effect resistance alleles combined with genomic selection for polygenic background for both the target and general adaptation traits might be fruitful for increasing FER resistance in maize.
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影响因子：
14.9
作者：
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