Genomic characterization and pathogenicity of a porcine hemagglutinating encephalomyelitis virus strain isolated in China.
Genomic characterization and pathogenicity of a porcine hemagglutinating encephalomyelitis virus strain isolated in China.
复制标题
中国分离的一株猪血凝性脑脊髓炎病毒株的基因组特征及致病性。
DOI：
10.1007/s11262-018-1591-y
复制
发表时间：
2018-10
期刊：
影响因子：
1.6
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Shi J;Zhao K;Lu H;Li Z;Lv X;Lan Y;Guan J;He W;Gao F
猪血凝性脑脊髓炎病毒（PHEV）是冠状病毒科β冠状病毒属的一种病毒，可通过外周神经系统侵入中枢神经系统，引起吸吮仔猪脑脊髓炎或呕吐和消耗病（VWD）。到目前为止，虽然报道的PHEV的完整核苷酸序列很少，但没有注释。本研究旨在阐明PHEV/2008菌株的基因组特征、系统发育和致病性。PHEV/2008菌株基因组全长30684 bp， G + C含量为37.27%。该基因组包括至少11个预测开放阅读框（orf），两侧分别为211和289个核苷酸的5 ‘和3 ’非翻译区（UTR）。复制酶多蛋白pp1a和pp1ab分别含有4382和7094个氨基酸残基，被两种病毒蛋白酶切割成16个亚基。基于全基因组序列的系统发育分析表明，PHEV/2008株在遗传上与其他已知PHEV型不同，为一种新的基因型（GI-1）。此外，我们发现PHEV/2008对4周龄BALB/c小鼠具有神经嗜性和高致病性。综上所述，这是中国第一个详细注释的PHEV新基因型菌株的完整基因组序列。
Porcine hemagglutinating encephalomyelitis virus (PHEV) is a member of the genus betacoronavirus within the family coronaviridae, which invades the central nervous system (CNS) via peripheral nervous system and causes encephalomyelitis or vomiting and wasting disease (VWD) in sucking piglets. Up to now, although few complete nucleotide sequences of PHEV have been reported, they are not annotated. This study aimed to illuminate genome characterization, phylogenesis and pathogenicity of the PHEV/2008 strain. The full length of the PHEV/2008 strain genome was 30,684 bp, with a G + C content of 37.27%. The genome included at a minimum of 11 predicted open reading frames (ORFs) flanked by 5′ and 3′ untranslated regions (UTR) of 211 and 289 nucleotides. The replicase polyproteins pp1a and pp1ab, which had 4382 and 7094 amino acid residues, respectively, were predicted to be cleaved into 16 subunits by two viral proteinases. Phylogenetic analysis based on the complete genome sequence revealed that PHEV/2008 strain was genetically different from other known PHEV types, which represented a novel genotype (GI-1). In addition, we found that PHEV/2008 was neurotropic and highly pathogenic to 4-week-old BALB/c mice. Taken together, this is the first detailed annotated, complete genomic sequence of a new genotype PHEV strain in China.
登录
查看更多内容
DOI：
10.1038/nrmicro2147
发表时间：
2009-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
0.8
作者：
Hirano N;Nomura R;Tawara T;Tohyama K
通讯作者：
Tohyama K
韩国猪血凝性脑脊髓炎病毒的检测和遗传分析。
影响因子：
1.6
作者：
Rho S;Moon HJ;Park SJ;Kim HK;Keum HO;Han JY;Van Nguyen G;Park BK
通讯作者：
Park BK
影响因子：
11.3
作者：
Sola I;Almazán F;Zúñiga S;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
5.4
作者：
Woo, PCY;Lau, SKP;Yuen, KY
通讯作者：
Yuen, KY