Neurotropism of swine haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon host age and route of infection.
Neurotropism of swine haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon host age and route of infection.
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DOI：
10.1016/s0021-9975(03)00083-5
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发表时间：
2004-01
影响因子：
0.8
通讯作者：
Tohyama K
中科院分区：
文献类型：
--
作者：
Hirano N;Nomura R;Tawara T;Tohyama K
用血凝性脑脊髓炎病毒(HEV)67N株经脑内(I.C.)、鼻腔(I.N.)、腹膜(I.P.)、皮下(S.C.)、静脉(IV.)途径接种1、4、8周龄小鼠。或口服途径，剂量不同。在一周大的小鼠中，无论接种途径如何，死亡率和平均死亡时间几乎相同，除了口服途径，似乎效果最差。该病毒通过除口服外的所有接种途径很容易杀死4周龄的小鼠，通过I.C.、I.N.或者南加州大学。接种疫苗。HEV感染途径由大到小依次为：I.C.>I.N.S.C.>I.P.>I.V.还有口交。为了跟踪HEV从周围神经向中枢神经系统(CNS)的传播，将病毒接种到4周龄小鼠的右后腿皮下。病毒最早在第2天在脊髓中检出，第3天在脑中检出。当动物出现中枢神经系统症状时，脑内滴度高于脊髓，最高可达107PFU/0.2g。免疫组织化学方法首先在同侧腰髓和背根神经节中检测到病毒抗原，随后在海马和大脑皮层的锥体细胞和小脑的浦肯野细胞中检测到病毒抗原，而在室管膜细胞、脉络丛细胞或其他神经胶质细胞中未检测到病毒抗原。感染神经元未见细胞病理学改变。
Mice aged 1, 4 or 8 weeks were inoculated with haemagglutinating encephalomyelitis virus (HEV), strain 67N, by the intracerebral (i.c.), intranasal (i.n.), intraperitoneal (i.p.), subcutaneous (s.c.), intravenous (i.v.) or oral route, with different doses. In 1-week-old mice, mortality and mean time to death were mostly the same regardless of the inoculation route, except for the oral route, which appeared to be the least effective. The virus killed 4-week-old mice readily by all routes of inoculation except the oral, and 8-week-old mice by i.c., i.n. or s.c. inoculation. In descending order of efficacy, the routes of HEV infection were: i.c., i.n., s.c., i.p., i.v. and oral. To follow the spread of HEV from peripheral nerves to the central nervous system (CNS), the virus was inoculated subcutaneously into the right hind leg of 4-week-old mice. The virus was first detected in the spinal cord on day 2, and in the brain on day 3. The brain titres became higher than those of the spinal cord, reaching a maximum of 107PFU/0.2 g when the animals were showing CNS signs. Viral antigen was first detected immunohistochemically in the lumbar spinal cord and the dorsal root ganglion ipsilateral to the inoculated leg; it was detected later in the pyramidal cells of the hippocampus and cerebral cortex, and in the Purkinje cells of the cerebellum but not in the ependymal cells, choroid plexus cells or other glial cells. The infected neurons showed no cytopathological changes.
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DOI：
10.1097/00005072-198911000-00005
发表时间：
1989-11-01
影响因子：
3.2
作者：
CARBONE, KM;TRAPP, BD;NARAYAN, O
通讯作者：
NARAYAN, O
影响因子：
0.8
作者：
YAGAMI, K;HIRAI, K;HIRANO, N
通讯作者：
HIRANO, N
影响因子：
0.8
作者：
Hirano N;Haga S;Sada Y;Tohyama K
通讯作者：
Tohyama K
DOI：
10.1097/00005072-196510000-00009
发表时间：
1965-01-01
影响因子：
3.2
作者：
JOHSNON, RT
通讯作者：
JOHSNON, RT
影响因子：
3.8
作者：
HIRANO, N;KAO, M;LUDWIG, H
通讯作者：
LUDWIG, H