SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway
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SPARCL1 通过 ITGB1 介导的信号通路影响牛骨骼肌衍生的卫星细胞迁移和分化
DOI：
10.3390/ani10081361
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发表时间：
2020-08
期刊：
影响因子：
3
通讯作者：
Huili Tong
中科院分区：
文献类型：
--
作者：
Yuxin Wang;Shuaiyu Liu;Yunqin Yan;Shufeng Li;Huili Tong
众所周知，细胞迁移和分化对于肌肉发育和肌肉疾病治疗具有非常重要而简单的基础。分泌型富含半胱氨酸的酸性蛋白样蛋白1（SPARCL 1）是细胞外基质的组成成分之一，对牛骨骼肌卫星细胞的分化具有调控作用。然而，确切的机制尚不清楚。这项研究揭示了SPARCL 1通过整合素β1促进肌源性卫星细胞的早期分化，从而为SPARCL 1在肌肉发育中的作用提供了新的见解。摘要SPARCL 1作为一种细胞外基质蛋白，参与多种细胞功能。它以前被牵连在牛骨骼肌衍生的卫星细胞（MDSC）的分化;然而，其潜在的机制仍然未知。在这项研究中，免疫沉淀和质谱分析显示，整合素β1（ITGB 1）与SPARCL 1结合。此外，免疫共沉淀证明SPARCL 1与ITGB 1相互作用。细胞划痕试验探索了SPARCL 1对MDSC通过ITGB 1迁移的影响。此外，肌管融合率和MyoD蛋白表达的结蛋白染色结果表明，SPARCL 1通过ITGB 1促进MDSC早期分化。此外，Western blotting结果表明SPARCL 1通过ITGB 1调节p-FAK、p-paxillin、vinculin、Cdc 42和Arp 2/3的表达。这些发现表明SPARCL 1可能通过ITGB 1介导的细胞信号通路影响牛MDSC迁移和分化。在此，我们阐明了SPARCL 1影响MDSC分化的机制。我们的研究结果提供了深入了解肌肉发育的分子机制，并可能在未来促进骨骼肌再生和治疗。
Simple Summary It is known that cell migration and differentiation have a very important yet simple basis for muscle development and muscle disease treatment. Secreted protein acidic and rich in cysteine like 1 (SPARCL1), one of the components of extracellular matrix, has been proved to regulate bovine skeletal muscle-derived satellite cell differentiation. However, the exact mechanism is not yet clear. This study reveals that SPARCL1 promotes muscle-derived satellite cell early differentiation through integrin β1, thereby providing a new insight into the role of SPARCL1 in muscle development. Abstract As an extracellular matrix protein, secreted protein acidic and rich in cysteine (SPARC)-like 1 (SPARCL1) is involved in various cell functions. It was previously implicated in bovine skeletal muscle-derived satellite cell (MDSC) differentiation; however, the underlying mechanism remains unknown. In this study, immunoprecipitation and mass spectrometry revealed that integrin β1 (ITGB1) combines with SPARCL1. Further, co-immunoprecipitation demonstrated that SPARCL1 interacts with ITGB1. Cell scratch assays explored the influence of SPARCL1 on MDSC migration through ITGB1. In addition, desmin staining for myotube fusion rate and MyoD protein expression results showed that SPARCL1 promotes MDSC early differentiation through ITGB1. Furthermore, Western blotting results demonstrated that SPARCL1 regulates the expression of p-FAK, p-paxillin, vinculin, Cdc42, and Arp2/3 through ITGB1. These findings indicate that SPARCL1 may influence bovine MDSC migration and differentiation through an ITGB1-mediated cell signaling pathway. Herein, we elucidated the mechanism through which SPARCL1 affects MDSC differentiation. Our results provide insight into the molecular mechanism of muscle development and may in the future facilitate skeletal muscle regeneration and treatment.
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DOI：
10.1111/febs.14195
发表时间：
2017-10
期刊：
The FEBS journal
影响因子：
--
作者：
Burridge K
通讯作者：
Burridge K
影响因子：
4.4
作者：
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通讯作者：
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影响因子：
5.8
作者：
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通讯作者：
Rudnicki, Michael A.
DOI：
10.1006/bbrc.2000.2682
发表时间：
2000-05-19
影响因子：
3.1
作者：
Cho, WJ;Kim, EJ;Lim, WK
通讯作者：
Lim, WK
影响因子：
1.8
作者：
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