Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus).
Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus).
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DOI：
10.1371/journal.pone.0253794
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Santangelo KS
中科院分区：
文献类型：
--
作者：
Spittler AP;Afzali MF;Bork SB;Burton LH;Radakovich LB;Seebart CA;Moore AR;Santangelo KS
Dunkin Hartley是生物医学研究中最常见的豚鼠品系，特别是用于哮喘，过敏，传染病，生殖和骨关节炎的研究。通常收集微创血液检查（例如全血细胞计数和血清生化谱）用于诊断和实验室分析。然而，尚未在该菌株中充分记录这些测定的参考区间。本研究旨在建立Dunkin Hartley豚鼠血液学和生化参数的参考区间，并确定年龄和性别相关差异。血液学和生化参数，回顾性地获得了145名男性和68名女性豚鼠之间的2和15个月的年龄。所有血液参数均由兽医临床病理学实验室进行分析。参考区间根据美国兽医临床病理学学会指南确定。使用非配对t检验或非参数Mann-Whitney检验确定年龄和性别相关差异。红细胞压积、红细胞分布宽度、平均血小板体积、白色血细胞计数、异嗜粒细胞、单核细胞、嗜酸性粒细胞、葡萄糖、血尿素氮、肌酐、钙、镁、总蛋白、白蛋白、球蛋白、胆固醇、天冬氨酸转氨酶、γ-谷氨酰转移酶和碳酸氢盐随年龄增长而增加。平均红细胞血红蛋白浓度、平均细胞血红蛋白浓度、血小板、淋巴细胞、磷、白蛋白/球蛋白比、碱性磷酸酶、阴离子间隙和计算的渗透压摩尔浓度随年龄增长而降低。与雌性动物相比，雄性动物的血红蛋白、红细胞压积、红细胞计数、平均红细胞血红蛋白浓度、白色血细胞计数、异嗜粒细胞、Foa-Kurloff细胞、丙氨酸氨基转移酶和碳酸氢盐较高，平均红细胞体积、红细胞分布宽度、血小板、平均血小板体积、嗜酸性粒细胞、总蛋白、白蛋白、球蛋白、胆固醇、钾、阴离子间隙、计算的渗透压摩尔浓度和铁较低。建立Dunkin Hartley豚鼠血液学和生化参数的年龄和性别差异，为其生理学提供了有价值的见解，以更好地评价诊断和实验结果。
The Dunkin Hartley is the most common guinea pig strain used in biomedical research, particularly for studies of asthma, allergy, infectious disease, reproduction, and osteoarthritis. Minimally invasive blood tests, such as complete blood counts and serum biochemistry profiles, are often collected for diagnostics and laboratory analyses. However, reference intervals for these assays have not yet been well-documented in this strain. The purpose of this study was to establish reference intervals for hematologic and biochemical parameters of Dunkin Hartley guinea pigs and determine age- and sex-related differences. Hematologic and biochemical parameters were retrospectively obtained from 145 male and 68 female guinea pigs between 2 and 15 months of age. All blood parameters were analyzed by a veterinary clinical pathology laboratory. Reference intervals were established according to the American Society for Veterinary Clinical Pathology guidelines. Age- and sex-related differences were determined using unpaired t-tests or nonparametric Mann-Whitney tests. Hematocrit, red blood cell distribution width, mean platelet volume, white blood cell count, heterophils, monocytes, eosinophils, glucose, blood urea nitrogen, creatinine, calcium, magnesium, total protein, albumin, globulin, cholesterol, aspartate aminotransferase, gamma glutamyl transferase, and bicarbonate increased with age. Mean corpuscular hemoglobin concentration, cellular hemoglobin concentration mean, platelets, lymphocytes, phosphorus, albumin/globulin ratio, alkaline phosphatase, anion gap, and calculated osmolality decreased with age. Males had higher hemoglobin, hematocrit, red blood cell count, mean corpuscular hemoglobin concentration, white blood cell count, heterophils, Foa-Kurloff cells, alanine aminotransferase, and bicarbonate and lower mean corpuscular volume, red blood cell distribution width, platelets, mean platelet volume, eosinophils, total protein, albumin, globulin, cholesterol, potassium, anion gap, calculated osmolality, and iron compared to females. Establishing age and sex differences in hematologic and biochemical parameters of Dunkin Hartley guinea pigs provides valuable insight into their physiology to better evaluate diagnostics and experimental results.
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影响因子：
2.4
作者：
LOVELL, DP;ARCHER, RK;MORGAN, RK
通讯作者：
MORGAN, RK
影响因子：
2.4
作者：
Seibell, J.;Bodie, K.;Blaich, G.
通讯作者：
Blaich, G.
影响因子：
2.5
作者：
Kile, BT;Mason-Garrison, CL;Justice, MJ
通讯作者：
Justice, MJ
DOI：
10.1016/0272-0590(86)90115-6
发表时间：
1986-11-01
期刊：
FUNDAMENTAL AND APPLIED TOXICOLOGY
影响因子：
--
作者：
SMITH, CN;NEPTUN, DA;IRONS, RD
通讯作者：
IRONS, RD
影响因子：
1.2
作者：
Waner, Trevor;Avidar, Yaakov;Bogin, Eitan
通讯作者：
Bogin, Eitan