Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition.
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition.
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DOI：
10.3791/56282
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发表时间：
2017-10-18
期刊：
影响因子：
--
通讯作者：
Blackband SJ
中科院分区：
文献类型：
--
作者：
Flint JJ;Menon K;Hansen B;Forder J;Blackband SJ
本方案描述了在收集磁共振（MR）显微镜数据期间支持急性脑切片制备物的正常代谢功能所需的程序。虽然有可能对活的、切除的哺乳动物组织进行MR收集，但这种实验传统上受到分辨率限制的限制，因此不能使组织微观结构可视化。相反，实现显微图像分辨率的MR协议需要使用固定的样本，以适应在漫长的扫描时间内静态、不变的条件的需要。目前的协议描述了第一个可用的MR技术，使活的，哺乳动物组织样本在显微镜分辨率成像。这样的数据是非常重要的，以了解发生在微观水平上的基于病理学的对比度变化如何影响宏观MR扫描的内容，如那些在临床上使用。一旦认识到这一点，就可以开发出灵敏度和准确性更高的诊断方法，这将直接转化为早期疾病治疗、更准确的治疗监测和改善患者预后。虽然所描述的方法集中于脑切片制备，但该方案适用于任何切除的组织切片，只要对气体和灌注液制备进行改变以适应组织的特定代谢需求。方案的成功执行应导致活的急性切片制备，其表现出长达15.5 h的MR扩散信号稳定性。当前系统优于其他MR兼容灌注设备的主要优点是其与获得更高分辨率图像所需的MR显微镜硬件的兼容性，以及在仔细调节灌注液条件下提供恒定、不间断流动的能力。减少的样品通量是这种设计的考虑因素，因为一次只能对一个组织切片进行成像。
This protocol describes the procedures necessary to support normal metabolic functions of acute brain slice preparations during the collection of magnetic resonance (MR) microscopy data. While it is possible to perform MR collections on living, excised mammalian tissue, such experiments have traditionally been constrained by resolution limits and are thus incapable of visualizing tissue microstructure. Conversely, MR protocols that did achieve microscopic image resolution required the use of fixed samples to accommodate the need for static, unchanging conditions over lengthy scan times. The current protocol describes the first available MR technique that enables imaging of living, mammalian tissue samples at microscopic resolutions. Such data is of great importance to the understanding of how pathology-based contrast changes occurring at the microscopic level influence the content of macroscopic MR scans such as those used in the clinic. Once such an understanding is realized, diagnostic methods with greater sensitivity and accuracy can be developed, which will translate directly to earlier disease treatment, more accurate therapy monitoring and improved patient outcomes. While the described methodology focuses on brain slice preparations, the protocol is adaptable to any excised tissue slice given that changes are made to the gas and perfusate preparations to accommodate the tissue's specific metabolic needs. Successful execution of the protocol should result in living, acute slice preparations that exhibit MR diffusion signal stability for periods up to 15.5 h. The primary advantages of the current system over other MR compatible perfusion apparatuses are its compatibility with the MR microscopy hardware required to attain higher resolution images and ability to provide constant, uninterrupted flow with carefully regulated perfusate conditions. Reduced sample throughput is a consideration with this design as only one tissue slice may be imaged at a time.
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影响因子：
5.7
作者：
Flint, Jeremy J.;Hansen, Brian;Portnoy, Sharon;Lee, Choong-Heon;King, Michael A.;Fey, Michael;Vincent, Franck;Stanisz, Greg J.;Vestergaard-Poulsen, Peter;Blackband, Stephen J.
通讯作者：
Blackband, Stephen J.
影响因子：
64.8
作者：
AGUAYO, JB;BLACKBAND, SJ;HINTERMANN, M
通讯作者：
HINTERMANN, M
影响因子：
4.6
作者：
Massin, C;Boero, C;Popovic, RS
通讯作者：
Popovic, RS
醛固定溶液改变了神经组织的水松弛和扩散特性。
影响因子：
3.3
作者：
Shepherd, Timothy M.;Thelwall, Peter E.;Stanisz, Greg J.;Blackband, Stephen J.
通讯作者：
Blackband, Stephen J.
影响因子：
2.5
作者：
KAMMAN R L;GO K G;BERENDSEN H J C
通讯作者：
BERENDSEN H J C