Sequencing and characterizing odorant receptors of the cerambycid beetle Megacyllene caryae.
Sequencing and characterizing odorant receptors of the cerambycid beetle Megacyllene caryae.
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DOI：
10.1016/j.ibmb.2012.03.007
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发表时间：
2012-07
影响因子：
3.8
通讯作者：
Robertson, Hugh M.
中科院分区：
文献类型：
--
作者：
Mitchell, Robert F.;Hughes, David T.;Luetje, Charles W.;Millar, Jocelyn G.;Soriano-Agaton, Flor;Hanks, Lawrence M.;Robertson, Hugh M.
气味受体是一类独特的配体门控离子通道，是昆虫识别挥发性化学物质的主要机制。在这里，我们描述了57个推定的ORS从触角转录组的天牛甲虫Megacyllene caryae（Gahan）测序。雄性甲虫产生的信息素混合物的9种成分，我们的功能特征ORS调谐到这些化学品中的三种：受体McOr 3是敏感的（S）-2-甲基-1-丁醇; McOr 20是敏感的（2S，3R）-2，3-己二醇;和McOr 5是敏感的2-苯基乙醇。McOr 3和McOr 20也对其他天牛的信息素结构相关的化学物质敏感，这表明orthopathy受体可能存在于许多天牛物种中。这些ORS是第一个从任何种类的甲虫的功能特点，并奠定了基础，了解内Cerambyorphae信息素的进化。
Odorant receptors (Ors) are a unique family of ligand-gated ion channels and the primary mechanism by which insects detect volatile chemicals. Here, we describe 57 putative Ors sequenced from an antennal transcriptome of the cerambycid beetle Megacyllene caryae (Gahan). The male beetles produce a pheromone blend of nine components, and we functionally characterized Ors tuned to three of these chemicals: receptor McOr3 is sensitive to (S)-2-methyl-1-butanol; McOr20 is sensitive to (2S,3R)-2,3-hexanediol; and McOr5 is sensitive to 2-phenylethanol. McOr3 and McOr20 are also sensitive to structurally-related chemicals that are pheromones of other cerambycid beetles, suggesting that orthologous receptors may be present across many cerambycid species. These Ors are the first to be functionally characterized from any species of beetle and lay the groundwork for understanding the evolution of pheromones within the Cerambycidae.
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影响因子：
2.3
作者：
Hanks, Lawrence M.;Millar, Jocelyn G.;Ray, Ann M.
通讯作者：
Ray, Ann M.
影响因子：
56.9
作者：
Nakagawa, T;Sakurai, T;Touhara, K
通讯作者：
Touhara, K
影响因子：
3.8
作者：
Engsontia, Patamarerk;Sanderson, Alan P.;Brown, Stephen
通讯作者：
Brown, Stephen
DOI：
10.1073/pnas.1017963108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Grosse-Wilde, Ewald;Kuebler, Linda S.;Hansson, Bill S.
通讯作者：
Hansson, Bill S.
影响因子：
2.2
作者：
Graham, Elizabeth E.;Mitchell, Robert F.;Hanks, Lawrence M.
通讯作者：
Hanks, Lawrence M.