Dipeptide-based polyphosphazene and polyester blends for bone tissue engineering.
Dipeptide-based polyphosphazene and polyester blends for bone tissue engineering.
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DOI：
10.1016/j.biomaterials.2010.02.058
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发表时间：
2010-06
期刊：
影响因子：
14
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Deng, Meng;Nair, Lakshmi S.;Nukavarapu, Syam R.;Jiang, Tao;Kanner, William A.;Li, Xudong;Kumbar, Sangamesh G.;Weikel, Arlin L.;Krogman, Nicholas R.;Allcock, Harry R.;Laurencin, Cato T.
关键词：
聚磷腈-聚酯共混物由于其具有无毒和中性pH降解产物的可控降解模式，是用于骨组织工程应用的有吸引力的材料。在我们正在进行的追求一个理想的完全可混溶的聚磷腈-聚酯共混体系，我们报告的合成和表征的混合取代基的可生物降解的聚磷腈聚[（甘氨酸乙基甘氨酸）1（苯基苯氧基）1磷腈]（PNEG/PhPh）及其与聚酯的共混物。以PNGEG/PhPh与聚（乳酸-乙醇酸）（PLAGA）的两种不同重量比制备两种基于二肽的共混物，即25：75（基质1）和50：50（基质2）。通过差示扫描量热法、傅里叶变换红外光谱和扫描电子显微镜证实了共混物的相容性。这两种共混物导致比聚酯更高的拉伸模量和强度。共混物在磷酸盐缓冲盐水中在37°C下在12周内显示出基质2 <基质1 < PLAGA的顺序的降解速率。与PLAGA相比，观察到混合物的降解介质的pH值显著更高，证实了聚磷腈水解产物对PLAGA酸性降解的中和作用。共混物组分PLAGA和聚磷腈表现出类似的降解模式，其特征在于分子量损失。此外，共混物表现出显着更高的成骨细胞生长速率相比，PLAGA，同时保持成骨细胞表型超过21天的文化。在大鼠皮下植入模型中，与PLAGA相比，两种混合物在12周内均表现出改善的生物相容性。
Polyphosphazene-polyester blends are attractive materials for bone tissue engineering applications due to their controllable degradation pattern with non-toxic and neutral pH degradation products. In our ongoing quest for an ideal completely miscible polyphosphazene-polyester blend system, we report synthesis and characterization of a mixed-substituent biodegradable polyphosphazene poly[(glycine ethyl glycinato)1(phenyl phenoxy)1phosphazene] (PNGEG/PhPh) and its blends with a polyester. Two dipeptide-based blends namely 25:75 (Matrix1) and 50:50 (Matrix2) were produced at two different weight ratios of PNGEG/PhPh to poly(lactic acid-glycolic acid) (PLAGA). Blend miscibility was confirmed by differential scanning calorimetry, Fourier transform infrared spectroscopy, and scanning electron microscopy. Both blends resulted in higher tensile modulus and strength than the polyester. The blends showed a degradation rate in the order of Matrix2 < Matrix1 < PLAGA in phosphate buffered saline at 37°C over 12 weeks. Significantly higher pH values of degradation media were observed for blends compared to PLAGA confirming the neutralization of PLAGA acidic degradation by polyphosphazene hydrolysis products. The blend components PLAGA and polyphosphazene exhibited a similar degradation pattern as characterized by the molecular weight loss. Furthermore, blends demonstrated significantly higher osteoblast growth rates compared to PLAGA while maintaining osteoblast phenotype over a 21-day culture. Both blends demonstrated improved biocompatibility in a rat subcutaneous implantation model compared to PLAGA over 12 weeks.
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DOI：
10.1007/s10904-006-9096-x
发表时间：
2006-12-01
影响因子：
4
作者：
El-Amin, Saadiq F.;Kwon, Michael S.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.
影响因子：
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作者：
ALLCOCK, HR;PUCHER, SR;SCOPELIANOS, AG
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SCOPELIANOS, AG
影响因子：
14
作者：
Deng, Meng;Nair, Lakshmi S.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.
DOI：
10.1073/pnas.0608837104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Cooper, James A., Jr.;Sahota, Janmeet S.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.
影响因子：
19
作者：
Kofron, Michelle D.;Griswold, Allison;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.