Microgravity inhibits resting T cell immunity in an exposure time-dependent manner.
Microgravity inhibits resting T cell immunity in an exposure time-dependent manner.
复制标题
微重力以暴露时间依赖性方式抑制静息 T 细胞免疫
DOI：
10.7150/ijms.7651
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发表时间：
2014
影响因子：
3.6
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Luo H;Wang C;Feng M;Zhao Y
背景：航天飞行后免疫功能下降是有据可查的。微重力是直接抑制免疫系统功能的关键因素之一。虽然微重力对T细胞免疫反应有抑制作用，但对静息状态的T淋巴细胞进行微重力预暴露是否会抑制T细胞的活化尚不清楚。方法：研究模拟微重力(MMG)预暴露0、8、16和24小时后静息的CD4+和CD8+T细胞对刀豆蛋白A(ConA)刺激的反应能力。用流式细胞仪检测免疫细胞表型和亚群。结果：与1g对照组相比，经MMG处理的T细胞CD25、CD69和CD71的表达、炎症细胞因子的分泌和细胞增殖均降低，且呈时间依赖关系。此外，短期MMG治疗引起的CD4+T细胞比CD8+T细胞更严重的增殖抑制。结论：MMG可直接影响静息T细胞亚群。在细胞增殖方面，CD4+T细胞对微重力抑制比CD8+T细胞更敏感。这些结果为研究MMG引起的T细胞功能缺陷提供了新的见解。
Background: Decline immune function is well documented after spaceflights. Microgravity is one of the key factors directly suppressing the function of immune system. Though T cell immune response was inhibited by microgravity, it is not clearly whether activation would be inhibited after a pre-exposure of microgravity on T lymphocytes at the resting state. Methods: We herein investigated the response ability of resting CD4+ and CD8+ T cells experiencing pre-exposure of modeled microgravity (MMg) for 0, 8, 16 and 24 hrs to concanavalin A (ConA) stimulation. The phenotypes and subsets of immune cells were determined by flow cytometry. Results: Both CD4+ and CD8+ T cells with an MMg pre-exposure exhibited decreased expressions of activation-markers including CD25, CD69 and CD71, inflammatory cytokine secretion and cell proliferation in response to ConA compared with T cells with 1g controls in an MMg exposure time- dependent manner. Moreover, short term MMg treatment caused more severe decreased proliferation in CD4+ T cells than in CD8+ T cells. Conclusions: MMg can directly impact on resting T cell subsets. CD4+ T cells were more sensitive to the microgravity inhibition than CD8+ T cells in respect of cell proliferation. These results offered new insights for the MMg-caused T cell functional defects.
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DOI：
10.1111/j.1365-2257.2006.00848.x
发表时间：
2007-08-01
影响因子：
3
作者：
Artac, H.;Coskun, M.;Yesilipek, A.
通讯作者：
Yesilipek, A.
DOI：
10.1016/s0162-3109(99)00123-x
发表时间：
1999-11-01
期刊：
IMMUNOPHARMACOLOGY
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DOI：
10.1042/bj20120625
发表时间：
2013-01-01
期刊：
The Biochemical journal
影响因子：
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作者：
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通讯作者：
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影响因子：
5.5
作者：
ALLEBBAN, Z;ICHIKI, AT;LANGE, RD
通讯作者：
LANGE, RD
影响因子：
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作者：
Parish, Christopher R;Glidden, Megan H;Warren, Hilary S
通讯作者：
Warren, Hilary S