Control efficacy of Bacillus thuringiensis and a new granulovirus isolate against Cydia pomonella in orchards

Control efficacy of Bacillus thuringiensis and a new granulovirus isolate against Cydia pomonella in orchards
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苏云金芽孢杆菌和一株新分离的颗粒病毒对果园苹果蠹蛾的防治效果

DOI:
10.1080/09583157.2013.791365
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发表时间:
2013-04
影响因子:
1.4
通讯作者:
Sun, Xiulian
Sun, Xiulian
中科院分区:
农林科学4区
文献类型:
--
作者:
Liu, Xiangyang;Lei, Chengfeng;Sun, Xiulian

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苹果蠹蛾是苹果和梨的重要害虫之一。本研究评价了颗粒体病毒、苏云金芽孢杆菌(Bt)菌株及其组合对黄瓜炭疽病的防治效果。中国的苹果蠹。从苹果蠹蛾(Cydia pomonella)体内分离到一株苹果蠹蛾颗粒体病毒(CpGV)。在中国甘肃的一个果园里发现了苹果蠹蛾的尸体。滴食法测定了CpGV分离株(CpGV-C1)对三龄幼虫的半数致死浓度(LC_(50))。苹果蠹蛾幼虫为770 OBs µl−1。Bt C-33和kurstaki HD-1对三龄幼虫的LC 50值分别为26.3 µg ml−1和15.7 µg ml−1。田间药效试验表明,CpGV-C1及其与Bt复配对黄瓜霜霉病具有较好的防治效果。苹果园内苹果蠹蛾幼虫与高效氯氰菊酯相似。说明CpGV与Bt联合应用可有效地保护苹果果实免受苹果炭疽病的危害。因此,有潜力开发一种低成本、高效的杀虫剂。
Abstract Codling moth, Cydia pomonella, is one of the most serious pests of apple and pear worldwide. This study evaluates the efficacies of a granulovirus, a Bacillus thuringiensis (Bt) strain and their combination in the control of C. pomonella in China. A Cydia pomonella granulovirus (CpGV) was isolated from C. pomonella cadavers in an orchard in Gansu, China. Droplet-feeding bioassays showed the median lethal concentration (LC50) of this CpGV isolate (CpGV-C1) against the third instar C. pomonella larvae was 770 OBs µl−1. The LC50 values of Bt C-33 and kurstaki HD-1 against the third instar larvae were 26.3 µg ml−1 and 15.7 µg ml−1, respectively. Field tests indicated the control efficacies of CpGV-C1 and the combination of CpGV-C1 and Bt against C. pomonella larvae in apple orchards were similar to that of beta-cypermethrin. Our data demonstrated that the combination of CpGV and Bt might effectively protect apple fruits from the damage of C. pomonella larvae and had the potential to be developed as a low-cost, highly effective insecticide.
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