The time-course of broiler intestinal microbiota development after administration of cecal contents to incubating eggs.
The time-course of broiler intestinal microbiota development after administration of cecal contents to incubating eggs.
复制标题
将盲肠内容物给予孵化蛋后肉鸡肠道微生物群发育的时间过程。
作者：
Donaldson EE;Stanley D;Hughes RJ;Moore RJ
众所周知，栖息在胃肠道 (GIT) 中的微生物种群会影响宿主的健康和生长性能。商业家禽业中的清洁孵化场和机器孵化实践可能会导致鸡的胃肠道中出现异常微生物群，并导致禽类之间出现高度变异。群体微生物特征的缺乏一致性给利用和操纵肠道细菌以更好地为宿主服务带来了挑战。在孵化前，使用来自高或低性能鸡的盲肠内容物接种鸡蛋表面，然后监测最初的肠道定植，并跟踪肠道微生物群组成随时间的变化。将两个不同的盲肠治疗组与未治疗的对照组进行比较 (n = 32)。使用高通量 16S rRNA 基因测序技术对细菌群落进行表征。通过鸡蛋表面应用进行的盲肠微生物群转移并没有将供体的性能特征转移给受体鸟类。其中一次盲肠接种提供了更均匀的肠道微生物群，但在使用不同接种物的第二组中并未重现这一点。肠道群落的发育在所有三组中都是可重复的，一些属（如乳杆菌）没有表现出任何变化，其他属（如粪杆菌属）随着时间的推移而丰度缓慢而稳定地增加，而其他属（如肠杆菌属）仅在生命的最初几天丰富。盲肠处理减少了禽类之间微生物群组成的差异。尽管供体鸡的高 FCR 性能并未随盲肠微生物群转移，但所有三组（包括对照组）的表现均优于该品种的标准。所有三个鸡群的微生物群发育模式相似，表明微生物群获取的正常过程在很大程度上淹没了应用于鸡蛋的盲肠材料的任何影响。
The microbial populations that inhabit the gastrointestinal tract (GIT) are known to influence the health and growth performance of the host. Clean hatcheries and machine-based incubation practices in the commercial poultry industry can lead to the acquisition of aberrant microbiota in the GIT of chickens and a very high level of bird-to-bird variation. The lack of microbial profile flock uniformity presents challenges for harnessing and manipulating intestinal bacteria to better serve the host. Cecal contents from high or low performing chickens were used to inoculate the surface of eggs prior to hatching and then the initial gut colonisation was monitored and subsequent changes in gut microbiota composition were followed over time. Two different cecal treatment groups were compared to an untreated control group (n = 32). Bacterial communities were characterised using high-throughput 16S rRNA gene sequencing techniques. Cecal microbiota transfer via egg surface application did not transfer the performance profile of the donors to the recipient birds. One of the cecal inoculations provided a more uniform gut microbiota, but this was not reproduced in the second group with a different inoculum. Development of the intestinal community was reproducible in all three groups with some genera like Lactobacillus showing no change, others like Faecalibacterium increased in abundance slowly and steadily over time and others like Enterobacter were abundant only in the first days of life. The cecal treatment reduced bird-to-bird variation in microbiota composition. Although the high FCR performance of donor birds was not transferred with the cecal microbiota, all three groups, including the control, performed better than standard for the breed. The pattern of microbiota development was similar in all three flocks, indicating that the normal processes of microbiota acquisition largely swamped any effect of the cecal material applied to eggs.
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影响因子：
5.7
作者：
Lagier JC;Million M;Hugon P;Armougom F;Raoult D
通讯作者：
Raoult D
影响因子：
5.2
作者：
Varmuzova K;Kubasova T;Davidova-Gerzova L;Sisak F;Havlickova H;Sebkova A;Faldynova M;Rychlik I
通讯作者：
Rychlik I
影响因子：
2
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Collado, MC;Gueimonde, M;Salminen, S
通讯作者：
Salminen, S
影响因子：
4.4
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Lu, JR;Idris, U;Lee, MD
通讯作者：
Lee, MD
影响因子：
4.4
作者：
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通讯作者：
Weightman, AJ