Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice.
Topographical distribution and morphology of SP-IR axons in the antrum, pylorus, and duodenum of mice.
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DOI：
10.1016/j.autneu.2023.103074
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发表时间：
2023-05
影响因子：
2.7
通讯作者：
Cheng, Zixi (Jack)
中科院分区：
文献类型：
--
作者：
Mistareehi, Anas;Bendowski, Kohlton T.;Bizanti, Ariege;Madas, Jazune;Zhang, Yuanyuan;Kwiat, Andrew M.;Nguyen, Duyen;Kogut, Nicole;Ma, Jichao;Chen, Jin;Cheng, Zixi (Jack)
关键词：
P物质（SP）是伤害性传入轴突常用的标志物，它在多种生理功能中发挥重要作用，包括对运动、肠道分泌和血管流量的调节。此前，我们发现SP免疫反应性（SP-IR）轴突密集地支配着小鼠全胃肌层平铺标本的幽门窦。然而，SP-IR轴突在黏膜下层和黏膜中的区域分布及形态尚未得到充分记录。在本研究中，将小鼠的胃窦 - 幽门 - 十二指肠（APD）进行横向和纵向切片。使用蔡司M2成像仪对每个APD的连续切片进行扫描（每个切片拼接图由50 - 100张全聚焦最大投影图像组成）。为确定SP-IR轴突及其终末的详细结构，我们使用共聚焦显微镜扫描感兴趣的区域。我们发现：1）SP-IR轴突支配肌层、黏膜下层和黏膜层。2）在肌层，SP-IR串珠样轴突密集地支配肌肉，并形成环绕肌间神经元的串珠样终末。3）在黏膜下层，SP-IR轴突支配血管和黏膜下神经节，并在布伦纳腺中形成网络。4）在黏膜层，SP-IR轴突支配黏膜肌层。一些SP-IR轴突进入固有层。5）胃窦和十二指肠的肌层SP-IR轴突密度高于幽门括约肌。6）SP-IR轴突有外在和内在两种来源。这项工作在单细胞/轴突/串珠水平上全面展示了APD中SP-IR轴突的分布和形态。这些数据将用于构建APD中SP-IR轴突支配的三维构架。
Substance-P (SP) is a commonly used marker of nociceptive afferent axons, and it plays an important role in a variety of physiological functions including the regulation of motility, gut secretion, and vascular flow. Previously, we found that SP-immunoreactive (SP-IR) axons densely innervated the pyloric antrum of the flat-mount of the mouse whole stomach muscular layer. However, the regional distribution and morphology of SP-IR axons in the submucosa and mucosa were not well documented. In this study, the mouse antrum-pylorus-duodenum (APD) were transversely and longitudinally sectioned. A Zeiss M2 imager was used to scan the serial sections of each APD (each section montage consisted of 50–100 all-in-focus maximal projection images). To determine the detailed structures of SP-IR axons and terminals, we used the confocal microscope to scan the regions of interest. We found that 1) SP-IR axons innervated the muscular, submucosal, and mucosal layers. 2) In the muscular layer, SP-IR varicose axons densely innervated the muscles and formed varicose terminals which encircled myenteric neurons. 3) In the submucosa, SP-IR axons innervated blood vessels and submucosal ganglia and formed a network in Brunner’s glands. 4) In the mucosa, SP-IR axons innervated the muscularis mucosae. Some SP-IR axons entered the lamina propria. 5) The muscular layer of the antrum and duodenum showed a higher SP-IR axon density than the pyloric sphincter. 6) SP-IR axons were from extrinsic and intrinsic origins. This work provided a comprehensive view of the distribution and morphology of SP-IR axons in the APD at single cell/axon/varicosity scale. This data will be used to create a 3D scaffold of the SP-IR axon innervation of the APD.
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DOI：
10.1007/bf00520326
发表时间：
1973-01-01
期刊：
ZEITSCHRIFT FUR ANATOMIE UND ENTWICKLUNGSGESCHICHTE
影响因子：
--
作者：
COSTA, M;FURNESS, JB
通讯作者：
FURNESS, JB
影响因子：
3.3
作者：
DOCKRAY, GJ;VAILLANT, C;WALSH, JH
通讯作者：
WALSH, JH
影响因子：
3.3
作者：
COSTA, M;CUELLO, AC;FRANCO, R
通讯作者：
FRANCO, R
影响因子：
29.4
作者：
BOSSHARD, A;CHERYCROZE, S;CHAYVIALLE, JA
通讯作者：
CHAYVIALLE, JA
影响因子：
2.3
作者：
Brehmer, Axel;Rupprecht, Holger;Neuhuber, Winfried
通讯作者：
Neuhuber, Winfried