Hydrocarbon-degrading bacteria in deep-water subarctic sediments (Faroe-Shetland Channel).
Hydrocarbon-degrading bacteria in deep-water subarctic sediments (Faroe-Shetland Channel).
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DOI：
10.1111/jam.14030
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发表时间：
2018-10
影响因子：
4
通讯作者：
Witte U
中科院分区：
文献类型：
--
作者：
Gontikaki E;Potts LD;Anderson JA;Witte U
本研究的目的是沿着法罗群岛-设得兰群岛海峡（FSC）的深度断面石油降解沉积物细菌的基线描述和生物标志物类群的识别，用于检测FSC沉积物中的石油污染。来自135、500和1000 m的石油降解沉积物细菌在以原油作为唯一碳源的培养物中富集（分别在12、5和0°C下）。使用依赖于培养物和不依赖于培养物（克隆文库）的技术研究富集的群落。测试分离的细菌菌株的烃降解能力。细菌分离株包括众所周知的石油降解分类群和首次报道的几种（Sulfitabacterium，Ahrensia，Belliella，Chryseobacterium）。Oceanoacellales和Alteromonadales在所有站点的克隆库中占主导地位，但在属水平上存在显着差异，特别是在浅水和深水冷水站之间。Alcanivorax构成64%的克隆在FSC 135，但在较深的站缺席。在500和1000 m处，假交替单胞菌和Oleispira占优势。Oleispira属在FSC深海沉积物中原油降解的早期阶段成为主要参与者，特别是在零度以下的温度下。这一发现为未来生物监测工具的研究提供了一个方向，用于检测FSC深处以及可能的高纬度寒冷沃茨中的低水平原油污染。FSC的石油和天然气勘探发生在>1000米的深度，但缺乏在发生石油泄漏时评估生态系统恢复到丸前条件所需的基线环境数据。这项研究将有助于我们的能力，以评估在FSC石油释放的影响，并指导该地区量身定制的生物修复策略的方向。
The aim of this study was the baseline description of oil‐degrading sediment bacteria along a depth transect in the Faroe‐Shetland Channel (FSC) and the identification of biomarker taxa for the detection of oil contamination in FSC sediments. Oil‐degrading sediment bacteria from 135, 500 and 1000 m were enriched in cultures with crude oil as the sole carbon source (at 12, 5 and 0°C respectively). The enriched communities were studied using culture‐dependent and culture‐independent (clone libraries) techniques. Isolated bacterial strains were tested for hydrocarbon degradation capability. Bacterial isolates included well‐known oil‐degrading taxa and several that are reported in that capacity for the first time (Sulfitobacter, Ahrensia, Belliella, Chryseobacterium). The orders Oceanospirillales and Alteromonadales dominated clone libraries in all stations but significant differences occurred at genus level particularly between the shallow and the deep, cold‐water stations. Alcanivorax constituted 64% of clones at FSC135 but was absent at deeper stations. Pseudoalteromonas and Oleispira dominated the bacterial community at 500 and 1000 m. The genus Oleispira emerged as a major player in the early stages of crude oil degradation in deep‐sea sediments of the FSC particularly at subzero temperatures. This finding is offering a direction for future research into biomonitoring tools for the detection of low levels of crude oil contamination in the deep FSC, and possibly high latitude cold waters in general. Oil and gas exploration in the FSC occurs at depths >1000 m but baseline environmental data necessary for the assessment of ecosystem recovery to prespill conditions in the event of an oil spill are lacking. This study will contribute to our ability to assess the impact of oil release in the FSC and guide the direction of bioremediation strategies tailored to the area.
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影响因子：
2.9
作者：
Deppe, U;Richnow, HH;Antranikian, G
通讯作者：
Antranikian, G
影响因子：
5.2
作者：
Kimes NE;Callaghan AV;Aktas DF;Smith WL;Sunner J;Golding B;Drozdowska M;Hazen TC;Suflita JM;Morris PJ
通讯作者：
Morris PJ
影响因子：
3.7
作者：
Brooks GR;Larson RA;Schwing PT;Romero I;Moore C;Reichart GJ;Jilbert T;Chanton JP;Hastings DW;Overholt WA;Marks KP;Kostka JE;Holmes CW;Hollander D
通讯作者：
Hollander D
DOI：
10.1099/00207713-48-4-1213
发表时间：
1998-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Bowman, JP;McCammon, SA;McMeekin, TA
通讯作者：
McMeekin, TA
影响因子：
11.4
作者：
Dubinsky, Eric A.;Conrad, Mark E.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.