Seroprevalence and genetic characterization of Toxoplasma gondii in three species of pet birds in China.
Seroprevalence and genetic characterization of Toxoplasma gondii in three species of pet birds in China.
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DOI：
10.1186/1756-3305-7-152
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发表时间：
2014-04-01
影响因子：
3.2
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Cong W;Meng QF;Song HQ;Zhou DH;Huang SY;Qian AD;Su C;Zhu XQ
弓形虫病由原生动物寄生虫弓形虫引起，是世界上最常见的人畜共患病之一，影响世界范围内广泛的温血哺乳动物和鸟类。然而，中国没有关于宠物鸟感染弓形虫的信息。因此，本研究旨在了解甘肃省中国宠物鸟体内弓形虫感染情况。2011年8月至2012年9月，在甘肃省中国西北部三个有代表性的行政区域采集宠物鸟(Carduelis Spinus、Alauda ulgula、Cocothraustes Migrator lus)血样687份，用改良凝集试验(MAT)检测弓形虫抗体。从血清阳性的宠物禽脑组织中提取基因组DNA，用半套式聚合酶链式反应扩增弓形虫B1基因，对阳性标本进行多位点聚合酶链式反应-限制性片段长度多态性分析。弓形虫血清总阳性率为11.21%(77/687)。弓形虫的血清阳性率最高(11.65%)，其次是华支睾吸虫(11.39%)和迁徙弓形虫(5.26%)，差异无统计学意义(P >； )。在77份DNA样本中，8份弓形虫B1基因阳性，4份显示完整的基因分型结果。只鉴定出一种基因型(II型变种：ToxoDB#3)。本次调查结果表明，甘肃省中国宠物鸟体内存在弓形虫感染。这些数据为控制宠物鸟感染弓形虫策略的实施提供了基本信息。据我们所知，这是第一份关于中国宠物鸟中弓形虫流行率和基因分型的报告。
Toxoplasmosis, caused by the protozoan parasite Toxoplasma gondii, is one of the most common zoonosis worldwide, affecting a wide range of warm-blooded mammals and birds worldwide. However, no information on T. gondii infection in pet birds in China is available. Therefore, this study was performed to determine the prevalence of T. gondii infection in pet birds in Gansu province, China. A total of 687 blood samples were collected from pet birds (Carduelis spinus, Alauda gulgula, Cocothraustes migratorlus) in three representative administrative regions in Gansu province, northwest China between August 2011 and September 2012 T. gondii antibodies were determined using the modified agglutination test (MAT). Genomic DNA was extracted from the brain tissues of seropositive pet birds and T. gondii B1 gene was amplified using a semi-nested PCR.DNA samples giving positive B1 amplification were then genetically characterized using multi-locus PCR-RFLP. The overall T. gondii seroprevalence was 11.21% (77/687). C. spinus had the highest T. gondii seroprevalence (11.65%), followed by A. arvensis (11.39%) and C. migratorlus (5.26%), these differences were not statistically significant (P > 0.05). Of 77 DNA samples, 8 were positive for the T. gondii B1 gene, four showed complete genotyping results. Only one genotype (the Type II variant: ToxoDB genotype #3) was identified. The results of the present survey indicated the presence of T. gondii infection in pet birds in Gansu province, China. These data provide base-line information for the execution of control strategies against T. gondii infection in pet birds. To our knowledge, this is the first report documenting the occurrence of T. gondii prevalence and genotype in pet birds in China.
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影响因子：
3.7
作者：
Wang L;Chen H;Liu D;Huo X;Gao J;Song X;Xu X;Huang K;Liu W;Wang Y;Lu F;Lun ZR;Luo Q;Wang X;Shen J
通讯作者：
Shen J
影响因子：
2.6
作者：
El Behairy, A. M.;Choudhary, S.;Dubey, J. P.
通讯作者：
Dubey, J. P.
影响因子：
1.9
作者：
Zhou, Peng;Zhang, Han;Zhu, Xing-Quan
通讯作者：
Zhu, Xing-Quan
影响因子：
3.2
作者：
Zhou P;Chen Z;Li HL;Zheng H;He S;Lin RQ;Zhu XQ
通讯作者：
Zhu XQ
影响因子：
3.2
作者：
Jiang HH;Huang SY;Zhou DH;Zhang XX;Su C;Deng SZ;Zhu XQ
通讯作者：
Zhu XQ