Overexpression of members of the microRNA-183 family is a risk factor for lung cancer: a case control study.
Overexpression of members of the microRNA-183 family is a risk factor for lung cancer: a case control study.
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DOI：
10.1186/1471-2407-11-393
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发表时间：
2011-09-15
期刊：
影响因子：
3.8
通讯作者：
Zhang YK
中科院分区：
文献类型：
--
作者：
Zhu W;Liu X;He J;Chen D;Hunag Y;Zhang YK
肺癌是全球癌症相关死亡的主要原因。早期发现被认为是肺癌治疗的关键。MicroRNAs （miRNAs）已显示出作为诊断和预后指标的前景。本研究旨在鉴定对肺癌患者具有诊断和预后价值的特异性mirna，并探讨mirna表达谱与患者生存的相关性。通过实时定量逆转录聚合酶链反应（RT-qPCR）检测了来自肺癌患者（原发癌和非癌组织和血清）的70对样本以及来自正常志愿者和肺癌细胞系的44份血清样本中miR-183家族成员（miR-96、miR-182和miR-183）的基因表达。通过log-rank和Cox回归分析检测组织、血清中mirna表达与患者总生存期的相关性。miR-183家族成员在肺癌肿瘤和血清中的表达水平高于正常对照组。miR-96在肿瘤中的表达与其在血清中的表达呈正相关。Log-rank和Cox回归分析显示，miR-183家族的肿瘤和血清mirna的高表达与肺癌患者的总体低生存率相关。我们的研究结果表明，miR-96、miR-182和miR-183在肿瘤和血清中的表达可能被认为是肺癌诊断和预后的潜在新生物标志物。
Lung cancer is the leading cause of cancer-related deaths worldwide. Early detection is considered critical for lung cancer treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival. Gene expression of members of the miR-183 family (miR-96, miR-182, and miR-183) were examined in 70 paired samples from lung cancer patients (primary cancer and non-cancerous tissues and sera), as well as 44 serum samples from normal volunteers and lung cancer cell lines by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). The correlation between the expression of miRNAs in tissues, sera, and patient overall survival were also examined by log-rank and Cox regression analysis. Expression levels of members of the miR-183 family in lung cancer tumor and sera were higher than that of their normal counterparts. The miR-96 expression in tumors was positively associated with its expression in sera. Log-rank and Cox regression analyses demonstrated that high expression of tumor and serum miRNAs of the miR-183 family were associated with overall poor survival in patients with lung cancer. Our results suggest that the expressions of miR-96, miR-182, and miR-183 in tumor and sera may be considered potential novel biomarkers for the diagnosis and prognosis of lung cancer.
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