Downregulated expression of peroxiredoxin 4 in granulosa cells from polycystic ovary syndrome.
Downregulated expression of peroxiredoxin 4 in granulosa cells from polycystic ovary syndrome.
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多囊卵巢综合征颗粒细胞中过氧化还原蛋白 4 的表达下调
DOI：
10.1371/journal.pone.0076460
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu JY
中科院分区：
文献类型：
--
作者：
Meng Y;Qian Y;Gao L;Cai LB;Cui YG;Liu JY
过氧化物氧还蛋白4（Peroxiredoxin 4，PRDX 4）是过氧化物氧还蛋白（Peroxiredoxin，PRDX）家族的成员，是典型的2-Cys型PRDX。PRDX 4监测细胞区室内的氧化负荷，并减少与氧化应激和细胞凋亡相关的过氧化氢和烷基氢过氧化物。PRDX 4等抗氧化剂可能促进卵泡发育，参与PCOS的病理生理过程。我们在前期的研究中发现PRDX 4在小鼠卵母细胞卵丘复合体中表达，并且PRDX 4可能与卵泡发育有关。在这项研究中，我们探讨了PRDX 4在人类卵泡中的表达和PRDX 4在PCOS病理生理学中的可能作用。我们的数据表明，PRDX 4主要在人卵巢颗粒细胞中表达。与对照组相比，PCOS组PRDX 4 mRNA水平和蛋白水平均降低。PRDX 4水平降低可能与PCOS病理生理过程中的氧化应激有关。PRDX 4在体内和体外成熟卵泡颗粒细胞中的表达均高于未成熟卵泡，提示PRDX 4可能与卵泡发育密切相关。总之，我们的研究结果可能为PCOS的病理生理机制提供新的线索，并可能使用抗氧化剂，如PRDX 4的治疗策略。
Peroxiredoxin 4 (PRDX4), a member of Peroxiredoxin (PRDX) family, is a typical 2-Cys PRDX. PRDX4 monitors the oxidative burden within cellular compartment and reduces hydrogen peroxide and alkyl hydroperoxide related to oxidative stress and apoptosis. Antioxidant, like PRDX4, may promote follicle development and participate in the pathophysiology of PCOS. In our previous study, we found that PRDX4 was expressed in mice oocyte cumulus oophorus complex, and that PRDX4 could be associated with follicle development. In this study, we explored the expression of PRDX4 in human follicles and possible role of PRDX4 in PCOS pathophysiology. Our data showed that PRDX4 was mainly expressed in granulosa cells in human ovaries. When compared to control group, both PRDX4 mRNA level and protein level decreased in PCOS group. The lowered levels of PRDX4 may relate to oxidative stress in the pathophysiologic progress of PCOS. Furthermore, expression of PRDX4 in the granulosa cells of in vivo or in vitro matured follicles was higher than that in immatured follicles, which suggested that PRDX4 may have a close relationship with follicular development. Altogether, our findings may provide new clues of the pathophysiologic mechanism of PCOS and potential therapeutic strategy using antioxidant, like PRDX4.
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影响因子：
3.7
作者：
Palande KK;Beekman R;van der Meeren LE;Beverloo HB;Valk PJ;Touw IP
通讯作者：
Touw IP
影响因子：
6.1
作者：
Fauser, BCJM;Chang, J;Lobo, R
通讯作者：
Lobo, R
影响因子：
5.8
作者：
Azziz, R;Woods, KS;Yildiz, BO
通讯作者：
Yildiz, BO
影响因子：
6.7
作者：
Nakahara, K;Saito, H;Hiroi, M
通讯作者：
Hiroi, M
影响因子：
4.6
作者：
Palacio, JR;Iborra, A;Martínez, P
通讯作者：
Martínez, P