Difference in Energy Metabolism of Annulus Fibrosus and Nucleus Pulposus Cells of the Intervertebral Disc.
Difference in Energy Metabolism of Annulus Fibrosus and Nucleus Pulposus Cells of the Intervertebral Disc.
复制标题
DOI：
10.1007/s12195-011-0164-0
复制
发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Huang, C. -Y. Charles
中科院分区：
文献类型：
--
作者：
Salvatierra, Jessica Czamanski;Yuan, Tai Yi;Fernando, Hanan;Castillo, Andre;Gu, Wei Yong;Cheung, Herman S.;Huang, C. -Y. Charles
关键词：
下腰痛与腰椎间盘退变有关。退化的主要迹象之一是无法维持细胞外基质的完整性。细胞外基质的合成与细胞内三磷酸腺苷(即能量)的产生密切相关。椎间盘由纤维环和髓核两个主要解剖区域组成，这两个区域在结构和成分上都不同，表明它们的细胞代谢也可能是不同的。本研究的目的是研究有无动态压迫的纤维环和髓核细胞的能量代谢，并检验这两种细胞类型之间的差异。取猪环和核组织，酶消化。分离细胞并将其包埋在琼脂糖构建体中。动态加载的样品在2 Hz和15%的应变下正弦位移4h，通过测量三磷酸腺苷的含量和释放、葡萄糖消耗和乳酸/一氧化氮的产生来分析细胞的能量代谢。对环状细胞和核细胞的这些测量结果进行了比较。环状细胞和核细胞表现出不同的代谢途径。核细胞在动态负荷前后的三磷酸腺苷含量较高，而环状细胞则有较高的乳酸产量和葡萄糖消耗。压缩增加了两种细胞的三磷酸腺苷的释放，并增加了环状细胞的能量产生。动态负荷影响了椎间盘细胞的能量代谢，对环状细胞的影响更大。
Low back pain is associated with intervertebral disc degeneration. One of the main signs of degeneration is the inability to maintain extracellular matrix integrity. Extracellular matrix synthesis is closely related to production of adenosine triphosphate (i.e. energy) of the cells. The intervertebral disc is composed of two major anatomical regions: annulus fibrosus and nucleus pulposus, which are structurally and compositionally different, indicating that their cellular metabolisms may also be distinct. The objective of this study was to investigate energy metabolism of annulus fibrosus and nucleus pulposus cells with and without dynamic compression, and examine differences between the two cell types. Porcine annulus and nucleus tissues were harvested and enzymatically digested. Cells were isolated and embedded into agarose constructs. Dynamically loaded samples were subjected to a sinusoidal displacement at 2 Hz and 15% strain for 4 h. Energy metabolism of cells was analyzed by measuring adenosine triphosphate content and release, glucose consumption, and lactate/nitric oxide production. A comparison of those measurements between annulus and nucleus cells was conducted. Annulus and nucleus cells exhibited different metabolic pathways. Nucleus cells had higher adenosine triphosphate content with and without dynamic loading, while annulus cells had higher lactate production and glucose consumption. Compression increased adenosine triphosphate release from both cell types and increased energy production of annulus cells. Dynamic loading affected energy metabolism of intervertebral disc cells, with the effect being greater in annulus cells.
登录
查看更多内容
影响因子：
2.8
作者：
Kandel, Rita;Roberts, Sally;Urban, Jill P. G.
通讯作者：
Urban, Jill P. G.
影响因子：
3
作者：
Bibby, SRS;Jones, DA;Urban, JPG
通讯作者：
Urban, JPG
影响因子：
3
作者：
BERNICK, S;CAILLIET, R
通讯作者：
CAILLIET, R
影响因子：
3
作者：
Hutton, WC;Elmer, WA;Hair, GA
通讯作者：
Hair, GA
影响因子：
2.8
作者：
Kasra, M;Goel, V;Buckwalter, J
通讯作者：
Buckwalter, J