Rice osa-miR171c Mediates Phase Change from Vegetative to Reproductive Development and Shoot Apical Meristem Maintenance by Repressing Four OsHAM Transcription Factors.
Rice osa-miR171c Mediates Phase Change from Vegetative to Reproductive Development and Shoot Apical Meristem Maintenance by Repressing Four OsHAM Transcription Factors.
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水稻 osa-miR171c 通过抑制四种 OsHAM 转录因子介导从营养发育到生殖发育的相变和芽顶端分生组织的维持
DOI：
10.1371/journal.pone.0125833
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Fan T;Li X;Yang W;Xia K;Ouyang J;Zhang M
从营养发育到生殖发育的相变是植物的关键发育步骤之一，它受到环境和内源因素的调节。这一相变过程涉及茎尖分生组织 (SAM) 特性、miRNA 和开花整合子的维持。在这里，我们报道 miRNA osa-miR171c 靶向四种 GRAS (GAI-RGA-SCR) 植物特异性转录因子（OsHAM1、OsHAM2、OsHAM3 和 OsHAM4）来控制水稻 (Oryza sativa) 的花转变和 SAM 不确定性的维持。我们鉴定了水稻 T-DNA 插入延迟抽穗 (dh) 突变体，其中 OsMIR171c 基因的表达上调。该突变体表现出多效性表型缺陷，包括特别延长的营养期、延迟的抽穗日期和更大的芽尖。平行表达分析表明，osa-miR171c 在花转变过程中控制茎尖四个 OsHAM 的表达变化，并对光做出反应。在dh突变体中，幼年期-成年期相变负调节因子osa-miR156的表达上调，开花整合子Hd3a和RFT1的表达受到抑制，参与维持SAM不确定性的FON4负调节因子的表达也受到抑制。根据这些数据，我们提出抑制 osa-miR171c 介导的 OsHAM 转录因子通过维持 SAM 不确定性和抑制开花整合子来调节从营养发育到生殖发育的相变。
Phase change from vegetative to reproductive development is one of the critical developmental steps in plants, and it is regulated by both environmental and endogenous factors. The maintenance of shoot apical meristem (SAM) identity, miRNAs and flowering integrators are involved in this phase change process. Here, we report that the miRNA osa-miR171c targets four GRAS (GAI-RGA-SCR) plant-specific transcription factors (OsHAM1, OsHAM2, OsHAM3, and OsHAM4) to control the floral transition and maintenance of SAM indeterminacy in rice (Oryza sativa). We characterized a rice T-DNA insertion delayed heading (dh) mutant, where the expression of OsMIR171c gene is up-regulated. This mutant showed pleiotropic phenotypic defects, including especially prolonged vegetative phase, delayed heading date, and bigger shoot apex. Parallel expression analysis showed that osa-miR171c controlled the expression change of four OsHAMs in the shoot apex during floral transition, and responded to light. In the dh mutant, the expression of the juvenile-adult phase change negative regulator osa-miR156 was up-regulated, expression of the flowering integrators Hd3a and RFT1 was inhibited, and expression of FON4 negative regulators involved in the maintenance of SAM indeterminacy was also inhibited. From these data, we propose that the inhibition of osa-miR171c-mediated OsHAM transcription factors regulates the phase transition from vegetative to reproductive development by maintaining SAM indeterminacy and inhibiting flowering integrators.
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