Multiple Modes of Nematode Control by Volatiles of Pseudomonas putida 1A00316 from Antarctic Soil against Meloidogyne incognita.
Multiple Modes of Nematode Control by Volatiles of Pseudomonas putida 1A00316 from Antarctic Soil against Meloidogyne incognita.
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DOI：
10.3389/fmicb.2018.00253
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhai Y;Shao Z;Cai M;Zheng L;Li G;Huang D;Cheng W;Thomashow LS;Weller DM;Yu Z;Zhang J
从南极土壤中分离到的恶臭假单胞菌1A00316具有杀线虫的潜力，可用于生物防治黑穗病；然而，菌株1A00316是否会产生挥发性有机化合物（VOCs），以及它们是否有可能用于生物防治M. incognita，目前尚不清楚。本研究采用三室培养皿和96孔培养皿对恶臭p.p . putida 1A00316培养滤液产生的挥发性有机化合物进行了体外实验研究。结果表明：1A00316菌株的培养滤液中卵的孵化率明显低于无菌蒸馏水和培养基中卵的孵化率；1A00316菌株的培养滤液中卵的孵化率明显低于无菌蒸馏水和培养基中卵的孵化率；采用固相微萃取-气相色谱-质谱联用（SPME-GC/MS）对恶臭假单胞菌1A00316挥发物进行分析，挥发物包括二甲基二硫化物、1-十一烯、2-壬酮、2-辛酮、(Z)-己烯-1-乙酸酯、2-十一烯酮和1-（乙基氧基）-十八烷。其中，二甲基二硫醚、2-壬烷酮、2-辛烷酮、(Z)-己烯-1-乙酸酯和2-十一烷酮在96孔培养板上直接接触对黑纹夜蛾J2幼虫有较强的杀线虫活性，只有2-十一烷酮具有熏蒸作用。此外，7种挥发性有机化合物通过直接接触和熏蒸均能抑制隐密夜蛾的卵孵化。在2%水琼脂培养皿中，这7种挥发性有机化合物均对小野田鼠J2幼虫有驱避作用。上述结果表明，菌株1A00316的挥发性有机化合物通过杀线虫、熏蒸和驱避作用在根结线虫发育的不同阶段发挥作用，具有开发成防治根结线虫多种模式的药剂的潜力。
Pseudomonas putida 1A00316 isolated from Antarctic soil showed nematicidal potential for biological control of Meloidogyne incognita; however, little was known about whether strain 1A00316 could produce volatile organic compounds (VOCs), and if they had potential for use in biological control against M. incognita. In this study, VOCs produced by a culture filtrate of P. putida 1A00316 were evaluated by in vitro experiments in three-compartment Petri dishes and 96-well culture plates. Our results showed that M. incognita juveniles gradually reduced their movement within 24–48 h of incubation with mortality ranging from 6.49 to 86.19%, and mostly stopped action after 72 h. Moreover, egg hatching in culture filtrates of strain 1A00316 was much reduced compared to that in sterile distilled water or culture medium. Volatiles from P. putida 1A00316 analysis carried out by solid-phase micro-extraction gas chromatography–mass spectrometry (SPME-GC/MS) included dimethyl-disulfide, 1-undecene, 2-nonanone, 2-octanone, (Z)-hexen-1-ol acetate, 2-undecanone, and 1-(ethenyloxy)-octadecane. Of these, dimethyl-disulfide, 2-nonanone, 2-octanone, (Z)-hexen-1-ol acetate, and 2-undecanone had strong nematicidal activity against M. incognita J2 larvae by direct-contact in 96-well culture plates, and only 2-undecanone acted as a fumigant. In addition, the seven VOCs inhibited egg hatching of M. incognita both by direct-contact and by fumigation. All of the seven VOCs repelled M. incognita J2 juveniles in 2% water agar Petri plates. These results show that VOCs from strain 1A00316 act on different stages in the development of M. incognita via nematicidal, fumigant, and repellent activities and have potential for development as agents with multiple modes of control of root-knot nematodes.
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