Uterine lymphatics: passage of ink and lymphoid cells from the rat's uterine wall and lumen.
Uterine lymphatics: passage of ink and lymphoid cells from the rat's uterine wall and lumen.
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子宫淋巴管：来自大鼠子宫壁和腔的墨水和淋巴细胞的通道。
DOI：
10.1095/biolreprod28.4.941
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发表时间：
1983
影响因子：
3.6
通讯作者：
Lande,IJ
中科院分区：
文献类型：
--
作者：
Head,JR;Lande,IJ
测定置于循环大鼠子宫壁和内腔中的墨汁和活淋巴样细胞的命运。注射到子宫肌层中的印度墨水迅速填充主要淋巴干，到达同侧肾和/或髂淋巴结，这取决于沿着子宫长度的接种部位。似乎没有其他腹部或胸部淋巴结受累。然而，注入子宫腔的墨水渗透子宫内膜间质非常缓慢，仅在引流结节中可见散在的含染料吞噬细胞。载有墨水的巨噬细胞在子宫内膜中保留至少数天。通过以下几种技术追踪同样注射的类肉瘤细胞：1）对组织进行闪烁计数，以检测51 Cr标记的细胞;2）放射自显影，以检测[3 H]尿苷标记的细胞;3）荧光显微镜检查，以检测冷冻组织中含荧光素的细胞; 4）引流淋巴结中的移植物抗宿主反应。所有技术都得到了相同的结果：注射到子宫肌层中的细胞迅速出现在引流淋巴结中，诱导移植物抗宿主反应，而放置在子宫腔中的细胞仅在区域淋巴结中发现少量，即使在几天后，移植物抗宿主反应也很小或不存在。细胞从子宫腔移动的屏障仍有待确定，但由于细胞向另一方向的运输是一个有充分记录的正常过程，因此相当令人感兴趣。限制细胞进入间质，巨噬细胞在该部位的长期滞留以及子宫内膜中淋巴管明显缺乏的综合作用最有可能是减缓来自该部位的抗原处理，这可能有助于调节妊娠期间的母体免疫应答。
The fate of ink and viable lymphoid cells placed in the wall and the lumen of the cycling rat uterus was determined. India ink injected into the myometrium rapidly filled major lymphatic trunks, going to the ipsilateral renal and/or iliac lymph nodes, depending on the site of inoculation along the uterus’ length. No other abdominal or thoracic nodes seem to be involved. However, ink injected into the uterine lumen penetrated the endometrial stroma very slowly and only scattered dye-containing phagocytic cells could be seen in draining nodes. Ink-laden macrophages were retained for at least several days in the endometrium. Lymphoid cells similarly injected were traced by several techniques:1) scintillation counting of tissues for51Cr-labeled cells;2) autoradiography for [3H]uridine-labeled cells;3) fluorescent microscopy for fluorescein-containing cells in frozen tissues; and4) graft-versus-host reactivity in draining lymph nodes. All techniques gave the same results: cells injected into the myometrium appeared rapidly in draining nodes, inducing a graft-versus-host reaction, whereas cells placed in the uterine lumen were found in only small numbers in the regional nodes, even after several days, and graft-versus-host reactions were minimal or absent. The barrier(s) to cell movement from the uterine lumen remains to be defined, but is of considerable interest since cell traffic in the other direction is a well-documented normal process. The combined effect of restricted cell entrance to the stroma, long retention of macrophages at this site, and an apparent paucity of lymphatic vessels in the endometrium most likely is to slow antigen handling from this site, which may help regulate maternal immune responses during pregnancy.
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影响因子：
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作者：
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