Gill Transcriptomic Responses to Toxin-producing Alga Prymnesium parvum in Rainbow Trout.
Gill Transcriptomic Responses to Toxin-producing Alga Prymnesium parvum in Rainbow Trout.
复制标题
DOI：
10.3389/fimmu.2021.794593
复制
发表时间：
2021
影响因子：
7.3
通讯作者：
Martin SAM
中科院分区：
文献类型：
--
作者：
Clinton M;Król E;Sepúlveda D;Andersen NR;Brierley AS;Ferrier DEK;Hansen PJ;Lorenzen N;Martin SAM
硬骨鱼的鳃是一个多功能器官，参与许多生理过程，包括通过鳃相关淋巴组织（GIALT）保护粘膜鳃表面免受病原体和其他环境抗原的侵害。与气候变化相关的现象，如有害藻华的频率和规模增加，对鳃功能造成额外的压力，导致鱼类死亡率增加和鱼类死亡。然而，由于缺乏在实验室条件下对硬骨鱼进行的高通量转录组学研究，赤潮引起的鳃损伤的分子基础在很大程度上仍然未知。我们用少年虹鳟鱼（虹鳟鱼mykiss）调查的鳃组织的转录组反应的两个（高和低）的亚致死密度的毒素生产的小Prymnesium parvum，在非暴露的控制鱼。暴露于P. parvum的时间（4-5 h）足以在暴露的鱼中识别出三种不同的表型反应，使我们能够专注于对P. parvum的常见鳃转录组反应，这些反应与剂量和表型无关。对常见差异表达基因（DEG）、典型途径、上游调节因子和下游效应的检查指向由急性期反应信号传导、IL-6信号传导、IL-10信号传导、PKR在干扰素诱导和抗病毒反应中的作用、IL-8信号传导和IL-17信号传导途径的改变驱动的小毕赤酵母诱导的炎症反应和鳃炎症。虽然我们无法确定推断的鳃炎是否正在进展或解决，但我们的研究清楚地表明，P. parvum开花可能导致鱼类严重的鳃疾病。通过提供对硬骨鱼中产生毒素的P. parvum的鳃转录组反应的见解，我们的研究为研究如何在HAB变得致命之前监测和减轻其毒性开辟了新的途径。
The gill of teleost fish is a multifunctional organ involved in many physiological processes, including protection of the mucosal gill surface against pathogens and other environmental antigens by the gill-associated lymphoid tissue (GIALT). Climate change associated phenomena, such as increasing frequency and magnitude of harmful algal blooms (HABs) put extra strain on gill function, contributing to enhanced fish mortality and fish kills. However, the molecular basis of the HAB-induced gill injury remains largely unknown due to the lack of high-throughput transcriptomic studies performed on teleost fish in laboratory conditions. We used juvenile rainbow trout (Oncorhynchus mykiss) to investigate the transcriptomic responses of the gill tissue to two (high and low) sublethal densities of the toxin-producing alga Prymnesium parvum, in relation to non-exposed control fish. The exposure time to P. parvum (4–5 h) was sufficient to identify three different phenotypic responses among the exposed fish, enabling us to focus on the common gill transcriptomic responses to P. parvum that were independent of dose and phenotype. The inspection of common differentially expressed genes (DEGs), canonical pathways, upstream regulators and downstream effects pointed towards P. parvum-induced inflammatory response and gill inflammation driven by alterations of Acute Phase Response Signalling, IL-6 Signalling, IL-10 Signalling, Role of PKR in Interferon Induction and Antiviral Response, IL-8 Signalling and IL-17 Signalling pathways. While we could not determine if the inferred gill inflammation was progressing or resolving, our study clearly suggests that P. parvum blooms may contribute to the serious gill disorders in fish. By providing insights into the gill transcriptomic responses to toxin-producing P. parvum in teleost fish, our research opens new avenues for investigating how to monitor and mitigate toxicity of HABs before they become lethal.
登录
查看更多内容
影响因子：
3.7
作者：
Dorantes-Aranda JJ;Seger A;Mardones JI;Nichols PD;Hallegraeff GM
通讯作者：
Hallegraeff GM
影响因子：
6.6
作者：
Binzer, Sofie Bjornholt;Svenssen, Daniel Killerup;Varga, Elisabeth
通讯作者：
Varga, Elisabeth
影响因子：
4.5
作者：
Bertin, Matthew J.;Voronca, Delia C.;Moeller, Peter D. R.
通讯作者：
Moeller, Peter D. R.
影响因子：
7.3
作者：
Barnabei L;Laplantine E;Mbongo W;Rieux-Laucat F;Weil R
通讯作者：
Weil R
二氧化锰纳米片在鱼鳃上皮细胞中诱导线粒体毒性。
影响因子：
5
作者：
Browning CL;Green A;Gray EP;Hurt R;Kane AB
通讯作者：
Kane AB