Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria.
Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria.
复制标题
尼日利亚Osun州牛肉屠宰场滋贺菌大肠杆菌的分子监测。
DOI：
10.1038/s41598-021-93347-w
复制
发表时间：
2021-07-07
影响因子：
4.6
通讯作者：
Happi C
中科院分区：
文献类型：
--
作者：
Ayoade F;Oguzie J;Eromon P;Omotosho OE;Ogunbiyi T;Olumade T;Akano K;Folarin O;Happi C
滋贺致突变株E.采用随机区组设计，在尼日利亚Osun州Osogbo大都会的选定屠宰场来源中筛选已知为腹泻病原体的大肠杆菌（STEC）的发病率/发生率。将样品直接铺在选择性和差异培养基上，并将E.大肠杆菌分离株。多重PCR分析用于筛选特异性毒力因子的存在。使用乳胶凝集血清分型试剂盒在血清学上确认这些为非O 157 STEC。仅出于鉴定目的，使用16 S基因对显示最高毒力基因组合的代表性分离株进行PCR产物的序列分析。结果表明，在Sekona-2屠宰板中采集的样品中的平均cfu/cm 2显著低于在Al-maleek分批屠宰场和Sekona-1屠宰板中采集的样品（按升序排列，P = 0.03）。此外，E.屠宰刀的大肠杆菌检出率显著低于工人手（P = 0.047）和屠宰场（P = 0.047），但与屠宰台（P = 0.98）和屠宰场废水（P = 0.39）无显著性差异。这些数据表明，屠宰场类型可能不像工人的卫生习惯那样重要，在STEC的流行和传播。对代表性分离株的序列分析显示，与来自加拿大的大肠杆菌O 113：H21（GenBank登录号：CP031892.1）菌株的覆盖率为100%，同源性为96.46%。该序列随后以登录号MW 463885提交给GenBank。从进化分析来看，本研究中测序的来自尼日利亚的菌株与来自尼日利亚的公开序列相比在进化上相距甚远。虽然没有E.虽然在研究区域内发现了O 157大肠杆菌，但在一些采样点，非O 157 STEC的发生率高达46.3%，这令人担忧，需要监管干预，以确保研究区域内屠宰场的卫生规范。
Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and E. coli isolates. Multiplex PCR analysis was used to screen for the presence of specific virulence factors. These were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes using the 16S gene for identification purposes only. Results showed that the average cfu/cm2 was significantly lower in the samples collected at Sekona-2 slaughter slab compared with those collected at Al-maleek batch abattoir and Sekona-1 slaughter slab in ascending order at P = 0.03. Moreover, the average cfu/cm2 E. coli in samples collected from butchering knife was significantly lower when compared with that of the workers’ hand (P = 0.047) and slaughtering floor (P = 0.047) but not with the slaughter table (P = 0.98) and effluent water from the abattoir house (P = 0.39). These data suggest that the abattoir type may not be as important in the prevalence and spread of STEC as the hygiene practices of the workers. Sequence analysis of a representative isolate showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. This sequence was subsequently submitted to GenBank with accession number MW463885. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.
登录
查看更多内容
影响因子：
5.5
作者：
Amézquita-López BA;Quiñones B;Soto-Beltrán M;Lee BG;Yambao JC;Lugo-Melchor OY;Chaidez C
通讯作者：
Chaidez C
影响因子：
5.5
作者：
Ranjbar R;Safarpoor Dehkordi F;Sakhaei Shahreza MH;Rahimi E
通讯作者：
Rahimi E
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
2.4
作者：
Akanbi, B. O.;Mbah, I. P.;Kerry, P. C.
通讯作者：
Kerry, P. C.
DOI：
10.3390/ijerph7103831
发表时间：
2010-10-01
影响因子：
--
作者：
Chigor, Vincent N.;Umoh, Veronica J.;Okoh, Anthony I.
通讯作者：
Okoh, Anthony I.