An in vitro model for the pathological degradation of articular cartilage in osteoarthritis.
An in vitro model for the pathological degradation of articular cartilage in osteoarthritis.
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DOI：
10.1016/j.jbiomech.2013.11.050
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发表时间：
2014-02-07
影响因子：
2.4
通讯作者：
Torzilli, Peter A.
中科院分区：
文献类型：
--
作者：
Grenier, Stephanie;Bhargava, Madhu M.;Torzilli, Peter A.
The objective of this study was to develop an in vitro cartilage degradation model that emulates the damage seen in early-stage osteoarthritis.为此，对软骨外植体进行胶原酶处理，以诱导关节表面胶原纤维和蛋白聚糖的酶降解。为了评估机械性能的变化，对完整和退化的软骨外植体进行了一系列有限压缩蠕变测试。 Changes in extracellular matrix structure and composition were determined using biochemical and histological approaches.我们的结果表明，胶原酶诱导的降解增加了软骨外植体在压缩下经历的变形量。 An increase in apparent permeability as well as a decrease in instantaneous and aggregate moduli were measured following collagenase treatment.降解外植体的组织学分析揭示了表面纤颤的存在、浅层和中间区域的蛋白聚糖耗尽以及芨芨草层的损失。 Collagen cleavage was confirmed by the Col II–¾Cshort antibody. Degraded specimens experienced a significant decrease in proteoglycan content but maintained total collagen content. Repetitive testing of degraded samples resulted in the gradual collapse of the articular surface and the compaction of the superficial zone.综上所述，我们的数据表明，胶原酶的酶促降解可用于模拟早期骨关节炎中观察到的变化。此外，我们的体外模型提供了有关软骨力学的信息以及有关基质变化如何影响软骨功能特性的见解。 More importantly, our model can be applied to develop and test treatment options for tissue repair.
The objective of this study was to develop an in vitro cartilage degradation model that emulates the damage seen in early-stage osteoarthritis. To this end, cartilage explants were collagenase-treated to induce enzymatic degradation of collagen fibers and proteoglycans at the articular surface. To assess changes in mechanical properties, intact and degraded cartilage explants were subjected to a series of confined compression creep tests. Changes in extracellular matrix structure and composition were determined using biochemical and histological approaches. Our results show that collagenase-induced degradation increased the amount of deformation experienced by the cartilage explants under compression. An increase in apparent permeability as well as a decrease in instantaneous and aggregate moduli were measured following collagenase treatment. Histological analysis of degraded explants revealed the presence of surface fibrillation, proteoglycan depletion in the superficial and intermediate zones and loss of the lamina splendens. Collagen cleavage was confirmed by the Col II–¾Cshort antibody. Degraded specimens experienced a significant decrease in proteoglycan content but maintained total collagen content. Repetitive testing of degraded samples resulted in the gradual collapse of the articular surface and the compaction of the superficial zone. Taken together, our data demonstrates that enzymatic degradation with collagenase can be used to emulate changes seen in early-stage osteoarthritis. Further, our in vitro model provides information on cartilage mechanics and insights on how matrix changes can affect cartilage’s functional properties. More importantly, our model can be applied to develop and test treatment options for tissue repair.
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影响因子：
2.8
作者：
AKIZUKI, S;MOW, VC;HOWELL, DS
通讯作者：
HOWELL, DS
DOI：
10.1302/0301-620x.58b1.131804
发表时间：
1976-01-01
影响因子：
--
作者：
MCDEVITT, CA;MUIR, H
通讯作者：
MUIR, H
DOI：
10.1007/bf02223293
发表时间：
1977-01-01
期刊：
CALCIFIED TISSUE RESEARCH
影响因子：
--
作者：
HERRING, GM
通讯作者：
HERRING, GM
DOI：
10.1016/j.berh.2009.08.008
发表时间：
2010-02-01
影响因子：
5.2
作者：
Englund, Martin
通讯作者：
Englund, Martin
影响因子：
2.8
作者：
AKIZUKI, S;MOW, VC;MANICOURT, DH
通讯作者：
MANICOURT, DH