Effects of designed PLLA and 50:50 PLGA scaffold architectures on bone formation in vivo.
Effects of designed PLLA and 50:50 PLGA scaffold architectures on bone formation in vivo.
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DOI：
10.1002/term.497
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发表时间：
2013-02
影响因子：
3.3
通讯作者：
Hollister, Scott J.
中科院分区：
文献类型：
--
作者：
Saito, Eiji;Liao, Elly E.;Hu, Wei-Wen;Krebsbach, Paul H.;Hollister, Scott J.
关键词：
可生物降解的多孔支架被认为是目前金属、陶瓷和聚合物骨移植替代丢失或受损骨组织的一种替代方法。虽然已有许多研究探讨了支架结构对骨形成的影响，但许多支架是通过盐析和相分离等传统方法制备的，并且没有设计好的结构。为了研究设计的结构和材料对骨形成的影响，我们利用基于图像的设计和间接固体自由构筑技术，设计并制备了两种可生物降解材料聚L-乳酸(PLLA)和50：50聚(乳酸-乙醇酸)(PLGA)三种多孔支架结构，将其接种于转导骨形态发生蛋白-7的人牙龈成纤维细胞，并将其植入小鼠皮下4周和8周。微计算机断层扫描数据证实，所制作的多孔支架复制了设计的结构。组织学分析显示，50：50PLGA支架在4周后降解，不再保持其结构。PLLA支架在两个时间点都保持了它们的结构，并显示出改善的骨生长，这与支架的内部结构一致。PLLA和50：50PLGA支架的力学性能均有所下降，但PLLA支架植入后的力学性能保持高于50：50PLGA。矿化组织的增多有助于支持4~8周的骨组织和支架结构的力学性能。结果表明，选择支架材料和计算机设计的支架以控制组织形成和力学性能以获得所需的骨组织再生是非常重要的。
Biodegradable porous scaffolds have been investigated as an alternative approach to current metal, ceramic, and polymer bone graft substitutes for lost or damaged bone tissues. Although there have been many studies investigating the effects of scaffold architecture on bone formation, many of these scaffolds were fabricated using conventional methods, such as salt leaching and phase separation, and were constructed without designed architecture. To study the effects of both designed architecture and material on bone formation, we designed and fabricated three types of porous scaffold architecture from two biodegradable materials, poly (L-lactic acid) (PLLA) and 50:50Poly (lactic-co-glycolic acid) (PLGA) using image based design and indirect solid freeform fabrication techniques, seeded them with bone morphogenic protein-7 transduced human gingival fibroblasts and implanted them subcutaneously into mice for 4 and 8 weeks. Micro-computed tomography data confirmed that the fabricated porous scaffolds replicated the designed architectures. Histological analysis revealed that the 50:50PLGA scaffolds degraded and did not maintain their architecture after 4 weeks. The PLLA scaffolds maintained their architecture at both time points and showed improved bone ingrowth which followed the internal architecture of the scaffolds. Mechanical properties of both PLLA and 50:50PLGA scaffolds decreased, but PLLA scaffolds maintained greater mechanical properties than 50:50PLGA after implantation. The increase of mineralized tissue helped to support mechanical properties of bone tissue and scaffold constructs from 4 to 8 weeks. The results indicated the importance of choice of scaffold materials and computationally designed scaffolds to control tissue formation and mechanical properties for desired bone tissue regeneration.
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影响因子：
14
作者：
Ishaug-Riley, SL;Crane-Kruger, GM;Mikos, AG
通讯作者：
Mikos, AG
DOI：
10.1034/j.1399-0020.2000.290115.x
发表时间：
2000-02-01
影响因子：
2.4
作者：
Hollister, SJ;Levy, RA;Feinberg, SE
通讯作者：
Feinberg, SE
影响因子：
4.9
作者：
Druecke, D;Langer, S;Homann, HH
通讯作者：
Homann, HH
影响因子：
2.4
作者：
Hui, PW;Leung, PC;Sher, A
通讯作者：
Sher, A
DOI：
10.1002/jbm.10050
发表时间：
2002-05-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Kohn, DH;Sarmadi, M;Krebsbach, PH
通讯作者：
Krebsbach, PH