Excavating differentially expressed antimicrobial peptides from transcriptome of Larimichthys crocea liver in response to Cryptocaryon irritans
Excavating differentially expressed antimicrobial peptides from transcriptome of Larimichthys crocea liver in response to Cryptocaryon irritans
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从大黄鱼肝脏转录组中挖掘响应白点虫刺激的差异表达抗菌肽
DOI：
10.1016/j.fsi.2018.01.028
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发表时间：
2018-04
影响因子：
4.7
通讯作者：
Hong Yu-cong
中科院分区：
文献类型：
--
作者：
Zheng Li-bing;Mao Yong;Wang Jun;Chen Ruan-ni;Su Yong-quan;Hong Yue-qun;Hong Yu-jian;Hong Yu-cong
大黄鱼是我国单一品种年产量最大的特种海洋经济鱼类。影响大黄鱼养殖的最重要因素之一是疾病，特别是由原虫隐核刺激物引起的海洋白斑病的威胁。抗菌肽（AMP）已被证明对细菌、真菌和寄生虫具有活性，显示出它们在水产养殖中作为抗生素替代品的潜在用途。关于AMPs的许多研究都集中在C上的活性抵抗上。 irritans 和 piscidin-like of L. crocea具有广泛的抗菌谱和较强的抗C. irritans 在我们团队中进行了筛选。文中利用大黄鱼肝脏转录组比较响应C．感染后3d，从差异表达基因中挖掘出7种AMP，包括LEAP2 like、LEAP-2A、hepcidin、hepcidin-like、piscidin-5-like、piscidin-5-like 4型和杀菌通透性增加蛋白（BPI）。 Hepcidin、hepcidin-like、piscidin-5-like、piscidin-5-like type4和BPI上调，以保护大黄鱼免受C.刺激感染；而LEAP2 like和LEAP-2A则被下调，它们可能作为负反馈调节因子或其他一些调节机制来调节C过程中的免疫反应。刺激感染。通过实时定量PCR（qRT-PCR）验证差异表达变化，以说明测序数据的可靠性。令人振奋的是，piscidin-5-like 4型是一种新型类型，与其他piscidin-5-like类型高度相似。有趣的是，感染很可能会导致 LEAP-2A mRNA 的选择性剪接，这是一个令人惊讶的现象，也是 C 后发现的。刺激感染，但还需要进行更多进一步的研究。因此，数据表明这些 AMP 参与了对 C 的免疫反应。刺激感染。总之，这些结果表明 AMP 对 C 的免疫反应。刺激感染是一个复杂而精密的监管过程。
Larimichthys crocea, the special marine economy fish, owns the largest annual yield for a single species in China. One of the most significant factors affecting large yellow croaker culture is the diseases, especially the threat of marine white spot disease which caused by a protozoanCryptocaryon irritans. Antimicrobial peptides (AMPs) have been demonstrated to be active against bacterium, fungi and parasites, showing their potential usefulness in aquaculture as substitutes for antibiotics. Many researches have been carried out about the AMPs concentrating on the activity resist onC. irritans, and piscidin-like ofL. croceaowning widely antibacterial spectrum and strong activity againstC. irritanswas screened in our team. In the paper, taking advantage of the large yellow croaker hepatic comparison transcriptome in response toC. irritansat 3d post infection, seven kinds of AMPs have been excavated from the differently expressed genes, including LEAP2 like, LEAP-2A, hepcidin, hepcidin-like, piscidin-5-like, piscidin-5-like type 4 and bactericidal permeability increasing protein (BPI). Hepcidin, hepcidin-like, piscidin-5-like, piscidin-5-like type4 and BPI were up-regulated to protect large yellow croaker from being damaged byC. irritansinfection; while LEAP2 like and LEAP-2A were down-regulated, they might be as a negative-feedback regulation factor or some other regulatory mechanisms to adjust the immune response in the process ofC. irritansinfection. The differential expression changes were verified with quantitative real-time PCR (qRT-PCR) to illustrate the reliability of the sequenced data. Hearteningly, piscidin-5-like type 4 was a novel type which was high similar to other piscidin-5-like types. Interestingly, the infection may well cause alternative splicing of LEAP-2A mRNA, which was a surprised phenomenon and finding afterC. irritansinfection, but more further study was needed to be conducted. Therefore, the data showed that these AMPs were involved in the immune response to theC. irritansinfection. In all, these results implied that the immune response of AMPs toC. irritansinfection was a complex and sophisticated regulatory process.
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影响因子：
4.7
作者：
Chen, Ruan-Ni;Su, Yong-Quan;Wu, Chang-Wen
通讯作者：
Wu, Chang-Wen
影响因子：
4.7
作者：
V. Rajanbabu;Jyh-Yih Chen
通讯作者：
V. Rajanbabu;Jyh-Yih Chen
影响因子：
2.9
作者：
Fernandes, JMO;Molle, G;Smith, VJ
通讯作者：
Smith, VJ
影响因子：
4.7
作者：
Xiang Zhang;Shaolin Wang;Songlin Chen;Ya-dong Chen;Yang Liu;C. Shao;Qilong Wang;Yang Lu;Guangye Gong;S. Ding;Z. Sha
通讯作者：
Xiang Zhang;Shaolin Wang;Songlin Chen;Ya-dong Chen;Yang Liu;C. Shao;Qilong Wang;Yang Lu;Guangye Gong;S. Ding;Z. Sha
影响因子：
2.9
作者：
Corrales, Jone;Mulero, Ivan;Noga, Edward J.
通讯作者：
Noga, Edward J.