The NLRP3-Mediated Neuroinflammatory Responses to CdTe Quantum Dots and the Protection of ZnS Shell
The NLRP3-Mediated Neuroinflammatory Responses to CdTe Quantum Dots and the Protection of ZnS Shell
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NLRP3 介导的 CdTe 量子点神经炎症反应和 ZnS 壳的保护
DOI：
10.2147/ijn.s246578
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发表时间：
2020-05
影响因子：
8
通讯作者：
Meng Tang
中科院分区：
文献类型：
--
作者：
Tianshu Wu;Xue Liang;Keyu He;Xi Liu;Yimeng Li;Yutong Wang;Lu Kong;Meng Tang
由于CdTe量子点（QD）仍然被广泛认为是先进的荧光探针，因为它们远优于非镉QD的上级光学性能和荧光效率，重要的是找到控制其毒性的方法。方法研究了两种含镉量子点（CdTe量子点和CdTe@ZnS量子点）对线虫C.通过对小鼠海马和培养的小胶质细胞的观察，探讨镉量子点引起的神经炎症反应及其可能的机制。结果首先，我们观察到含镉QD染毒可引起线虫的免疫反应和神经行为缺陷。优雅在用QD治疗的小鼠中，海马中对QD的神经炎症反应，包括小胶质细胞活化和IL-1 β释放，也发生了。在研究含镉量子点引起IL-1 β介导的炎症的机制时，发现含镉量子点通过引起过量ROS产生激活NLRP 3炎性体，并导致IL-1 β释放。讨论尽管与CdTe量子点相比，CdTe@ZnS量子点的免疫反应和神经毒性较轻，表明ZnS涂层具有保护作用，但NLRP 3表达和ROS产生的抑制完全减少了IL-1 β介导的炎症。 这提供了有价值的信息，即抑制靶分子是减轻含镉量子点毒性的有效和高效的方法，因此通过基于机制的风险评估来评估量子点是重要的。
Introduction Since CdTe quantum dots (QDs) are still widely considered as advanced fluorescent probes because of their far superior optical performance and fluorescence efficiency over non-cadmium QDs, it is important to find ways to control their toxicity. Methods In this study, the adverse effects of two cadmium-containing QDs, ie, CdTe QDs and CdTe@ZnS QDs, on the nervous system of nematode C. elegans, the hippocampus of mice, and cultured microglia were measured in order to evaluate the neuroinflammation caused by cadmium-containing QDs and the potential mechanisms. Results Firstly, we observed that cadmium-containing QD exposure-induced immune responses and neurobehavioral deficit in nematode C. elegans. In the mice treated with QDs, neuroinflammatory responses to QDs in the hippocampus, including microglial activation and IL-1ß release, occurred as well. When investigating the mechanisms of cadmium-containing QDs causing IL-1ß-mediated inflammation, the findings suggested that cadmium-containing QDs activated the NLRP3 inflammasome by causing excessive ROS generation, and resulted in IL-1ß release. Discussion Even though the milder immune responses and neurotoxicity of CdTe@ZnS QDs compared with CdTe QDs indicated the protective role of ZnS coating, the inhibitions of NLRP3 expression and ROS production completely reduced the IL-1ß-mediated inflammation. This provided valuable information that inhibiting target molecules is an effective and efficient way to alleviate the toxicity of cadmium-containing QDs, so it is important to evaluate QDs through a mechanism-based risk assessment.
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影响因子：
9.9
作者：
Bujnakova, Zdenka;Balaz, Matej;Psotka, Miroslav
通讯作者：
Psotka, Miroslav
影响因子：
8.9
作者：
Takafumi Kimura;T. Takanami;T. Sakashita;S. Wada;Y. Kobayashi;A. Higashitani
通讯作者：
Takafumi Kimura;T. Takanami;T. Sakashita;S. Wada;Y. Kobayashi;A. Higashitani
影响因子：
10.4
作者：
Patchin ES;Anderson DS;Silva RM;Uyeminami DL;Scott GM;Guo T;Van Winkle LS;Pinkerton KE
通讯作者：
Pinkerton KE
影响因子：
3.8
作者：
Park HH;Jung Y;Lee SV
通讯作者：
Lee SV
DOI：
10.1002/bio.3193
发表时间：
2017-05
期刊：
Luminescence : the journal of biological and chemical luminescence
影响因子：
--
作者：
D. Saikia;S. Chakravarty;N. S. Sarma;S. Bhattacharjee;P. Datta;N. C. Adhikary
通讯作者：
D. Saikia;S. Chakravarty;N. S. Sarma;S. Bhattacharjee;P. Datta;N. C. Adhikary