Blood-feeding induces reversible functional changes in flight muscle mitochondria of Aedes aegypti mosquito.
Blood-feeding induces reversible functional changes in flight muscle mitochondria of Aedes aegypti mosquito.
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DOI：
10.1371/journal.pone.0007854
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发表时间：
2009-11-16
期刊：
影响因子：
3.7
通讯作者：
Oliveira MF
中科院分区：
文献类型：
--
作者：
Gonçalves RL;Machado AC;Paiva-Silva GO;Sorgine MH;Momoli MM;Oliveira JH;Vannier-Santos MA;Galina A;Oliveira PL;Oliveira MF
噬血性对食血生物提出了挑战，因为血液消化的产物会对细胞产生有害影响。线粒体在细胞生物学中扮演多种角色，作为有氧能量转导途径的位点，也是活性氧（ROS）的重要来源，调节氧化还原代谢。因此，线粒体功能的调节应该是相关的吸血节肢动物。在这里，我们研究了吸血对埃及伊蚊（登革热和黄热病的传播媒介）的飞行肌（FM）线粒体的影响。吸血导致线粒体耗氧量的可逆性减少，这是一个与血液消化平行的事件。这些变化在血餐（ABM）后24小时最强烈，血液消化的高峰，当氧消耗被抑制68%。细胞色素c和a+ a3水平及电子传递链细胞色素c氧化酶活性在24h ABM时均降低。FM的超微结构和分子分析显示，线粒体融合后的血粉，一个条件有关减少ROS的产生。一致的是，BF诱导可逆的减少线粒体H2O2的形成在血液消化过程中，达到最低值在24小时ABM，其中观察到的减少51%。吸血引发了吸血昆虫线粒体的功能和结构变化，这可能是对吸血的一种重要适应。
Hematophagy poses a challenge to blood-feeding organisms since products of blood digestion can exert cellular deleterious effects. Mitochondria perform multiple roles in cell biology acting as the site of aerobic energy-transducing pathways, and also an important source of reactive oxygen species (ROS), modulating redox metabolism. Therefore, regulation of mitochondrial function should be relevant for hematophagous arthropods. Here, we investigated the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and yellow fever. Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an event that was parallel to blood digestion. These changes were most intense at 24 h after blood meal (ABM), the peak of blood digestion, when oxygen consumption was inhibited by 68%. Cytochromes c and a+a 3 levels and cytochrome c oxidase activity of the electron transport chain were all reduced at 24 h ABM. Ultrastructural and molecular analyses of FM revealed that mitochondria fuse upon blood meal, a condition related to reduced ROS generation. Consistently, BF induced a reversible decrease in mitochondrial H2O2 formation during blood digestion, reaching their lowest values at 24 h ABM where a reduction of 51% was observed. Blood-feeding triggers functional and structural changes in hematophagous insect mitochondria, which may represent an important adaptation to blood feeding.
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卡路里不能通过果蝇饮食限制来解释寿命的延长。
影响因子：
9.8
作者：
Mair, William;Piper, Matthew D W;Partridge, Linda
通讯作者：
Partridge, Linda
DOI：
10.1111/j.1749-6632.2002.tb02094.x
发表时间：
2002-01-01
期刊：
INCREASING HEALTHY LIFE SPAN: CONVENTIONAL MEASURES AND SLOWING THE INNATE AGING PROCESS
影响因子：
--
作者：
Lenaz, G;Bovina, C;Ventura, B
通讯作者：
Ventura, B
影响因子：
4.8
作者：
DANSAPETRETSKI, M;RIBEIRO, JMC;OLIVEIRA, PL
通讯作者：
OLIVEIRA, PL
影响因子：
4.1
作者：
AVIDOR, Y;GONDA, O
通讯作者：
GONDA, O
DOI：
10.1093/gerona/61.1.36
发表时间：
2006-01-01
影响因子：
5.1
作者：
Magwere, T;Goodall, S;Partridge, L
通讯作者：
Partridge, L