Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol.
Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol.
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Nocardioides sp。菌株WSN05-2，从小麦场分离出来，降解脱氧烯醇，产生新型的中间3-EPI-脱氧烯醇。
DOI：
10.1007/s00253-010-2857-z
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发表时间：
2011-01
影响因子：
5
通讯作者：
Tsushima, Seiya
中科院分区：
文献类型：
--
作者：
Ikunaga, Yoko;Sato, Ikuo;Grond, Stephanie;Numaziri, Nobutaka;Yoshida, Shigenobu;Yamaya, Hiroko;Hiradate, Syuntaro;Hasegawa, Morifumi;Toshima, Hiroaki;Koitabashi, Motoo;Ito, Michihiro;Karlovsky, Petr;Tsushima, Seiya
真菌毒素脱氧雪腐镰刀菌烯醇（DON）由于其对人类和牲畜的毒性，在世界范围内对小麦和大麦等作物的生产造成严重问题。采用富集培养的方法，从麦田土壤样品中筛选出一株能降解DON的细菌。命名为菌株WSN 05 -2的分离的细菌在孵育10天后从培养基中完全去除1，000 μg/mL的DON。系统发育分析表明，WSN 05 -2属于类诺卡氏菌属（Nocardioides）。WSN 05 -2在以DON为唯一碳源的培养基中生长显著。高效液相色谱分析表明，存在的主要初始代谢产物的DON的培养上清液。经质谱、核磁共振氢谱和碳谱分析，确定其代谢产物为3-epi-deoxynivalenol（3-epi-DON）。接种WSN 05 -2后第7天，小麦籽粒中DON的含量减少了约90%。这是首次报道能够降解DON的类诺卡氏菌属菌株和作为微生物降解DON的中间体的未知3-epi-DON。
The mycotoxin deoxynivalenol (DON) causes serious problems worldwide in the production of crops such as wheat and barley because of its toxicity toward humans and livestock. A bacterial culture capable of degrading DON was obtained from soil samples collected in wheat fields using an enrichment culture procedure. The isolated bacterium, designated strain WSN05-2, completely removed 1,000 μg/mL of DON from the culture medium after incubation for 10 days. On the basis of phylogenetic studies, WSN05-2 was classified as a bacterium belonging to the genus Nocardioides. WSN05-2 showed significant growth in culture medium with DON as the sole carbon source. High-performance liquid chromatography analysis indicated the presence of a major initial metabolite of DON in the culture supernatant. The metabolite was identified as 3-epi-deoxynivalenol (3-epi-DON) by mass spectrometry and 1H and 13C nuclear magnetic resonance analysis. The amount of DON on wheat grain was reduced by about 90% at 7 days after inoculation with WSN05-2. This is the first report of a Nocardioides sp. strain able to degrade DON and of the yet unknown 3-epi-DON as an intermediate in the degradation of DON by a microorganism.
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影响因子：
6.1
作者：
KING, RR;MCQUEEN, RE;GREENHALGH, R
通讯作者：
GREENHALGH, R
影响因子：
2.8
作者：
Goswami, Rubella S.;Xu, Jin-Rong;Kistler, H. Corby
通讯作者：
Kistler, H. Corby
DOI：
10.1080/02652039309374143
发表时间：
1993-03-01
期刊：
FOOD ADDITIVES AND CONTAMINANTS
影响因子：
--
作者：
ONEILL, K;DAMOGLOU, AP;PATTERSON, MF
通讯作者：
PATTERSON, MF
DOI：
10.1080/02652030110052283
发表时间：
2001-11-01
期刊：
FOOD ADDITIVES AND CONTAMINANTS
影响因子：
--
作者：
Lauren, DR;Smith, WA
通讯作者：
Smith, WA
影响因子：
4.4
作者：
Shima, J;Takase, S;Ochi, K
通讯作者：
Ochi, K