Selective, sensitive, and fast determination of S-layer proteins by a molecularly imprinted photonic polymer coated film and a fiber-optic spectrometer
Selective, sensitive, and fast determination of S-layer proteins by a molecularly imprinted photonic polymer coated film and a fiber-optic spectrometer
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通过分子印迹光子聚合物涂层薄膜和光纤光谱仪选择性、灵敏且快速地测定 S 层蛋白
DOI：
10.1007/s00216-019-02109-1
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发表时间：
2019-11
影响因子：
4.3
通讯作者：
Yuxing Guo
中科院分区：
文献类型：
--
作者：
Daodong Pan;Mingyue Xun;Hangzhen Lan;Jianlin Li;Zhen Wu;Yuxing Guo
采用胶体晶体模板法和表面分子印迹法制备了分子印迹光子聚合物（MIPP）膜，并将其用于选择性捕获复杂嗜酸乳杆菌样品中的S层蛋白（SLP）。胶体晶体模板通过浸渍过程形成，然后通过化学结合的印迹模板SLP分子。在用聚（甲基丙烯酸甲酯）载玻片覆盖SLP-二氧化硅层后，形成由两个载玻片组成的夹层结构。在用预先制备的聚合溶液聚合SLP-二氧化硅层之后，氢氟酸和乙酸磷酸盐缓冲溶液分别除去二氧化硅颗粒和SLP分子。所获得的MIPP膜表现出三维的、高度有序的和互连的大孔结构（孔径大于200 nm），这是SLP分子特别容易接近的。吸附的SLP分子简单，直接检测的光纤光谱仪。MIPP膜的布拉格衍射峰的红移与在膜中已经收获的SLP分子的数目线性相关。的SLP-MMIP-光纤光谱仪方法SLP的检测限为1 ng mL-1。该传感器成功地应用于检测粗提嗜酸乳杆菌样品中的SLP分子。我们的研究结果证明了SLP-MIPP薄膜用于快速实时测量SLP的适用性。图形摘要
A newly developed molecularly imprinted photonic polymer (MIPP) film, which was prepared by colloidal crystal templating and surface molecular imprinting, was used for selective capture of S-layer protein (SLP) from a complexLactobacillus acidophilussample. The colloidal crystal templates were formed by a dipping process followed by chemical binding of the imprinted template SLP molecules. A sandwich structure consisting of two glass slides was formed after the SLP–silica layer had been covered with a poly(methyl methacrylate) glass slide. After polymerization of the SLP–silica layer with the preprepared polymerization solution, hydrofluoric acid and acetic phosphate buffer solutions removed the silica particles and SLP molecules, respectively. The MIPP film obtained exhibited a three-dimensional, highly ordered and interconnected macroporous structure (pore size greater than 200 nm), which is specifically accessible to SLP molecules. The adsorbed SLP molecules were simply and straightforwardly detected by a fiber-optic spectrometer. The redshift of the Bragg diffraction peak of the MIPP film was linearly related to the number of SLP molecules that had been harvested in the film. The detection limit of the SLP–MMIP–fiber-optic spectrometer method for SLP was 1 ng mL-1. The MIPP sensor was successfully applied to detect SLP molecules in a crudely extractedLactobacillus acidophilussample. Our results prove the applicability of the SLP–MIPP film for fast and real-time measurement of SLP.Graphical abstract
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影响因子：
8.4
作者：
Yin-Xia Zhang;Pinwen Zhao;Li‐Ping Yu
通讯作者：
Yin-Xia Zhang;Pinwen Zhao;Li‐Ping Yu
影响因子：
64.8
作者：
Jeong, H;Mason, SP;Oltvai, ZN
通讯作者：
Oltvai, ZN
基于分子印迹光子水凝胶的痕量胆固醇无标记比色检测
影响因子：
--
作者：
Li, Jinhua;Zhang, Zhong;Peng, Hailong
通讯作者：
Peng, Hailong
DOI：
10.1002/chin.201123280
发表时间：
2011-06
期刊：
ChemInform
影响因子：
--
作者：
M. Whitcombe;I. Chianella;L. Larcombe;S. Piletsky;J. Noble;R. Porter;Adrian Horgan
通讯作者：
M. Whitcombe;I. Chianella;L. Larcombe;S. Piletsky;J. Noble;R. Porter;Adrian Horgan
影响因子：
5
作者：
Kadhem, Abbas J.;Xiang, Shuting;de Cortalezzi, Maria Fidalgo
通讯作者：
de Cortalezzi, Maria Fidalgo