Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen.
Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen.
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DOI：
10.1038/s41467-020-19347-y
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发表时间：
2020-11-02
影响因子：
16.6
通讯作者：
Pelicic V
中科院分区：
文献类型：
--
作者：
Muir A;Gurung I;Cehovin A;Bazin A;Vallenet D;Pelicic V
脑膜炎奈瑟菌会导致危及生命的脑膜炎和败血症。在这里，我们构建了一个完整的集合定义突变的蛋白质编码基因的有机体，确定所有的基因是必要的，在实验室条件下。这个名为NeMeSys 2.0的集合包含了1584个非必需基因的单个突变体。我们鉴定出391个必需基因，这些基因与基因组信息的表达和保存、细胞膜结构和功能以及代谢等基本功能有关。我们使用这个集合来阐明不同基因的功能，包括编码以前未被识别的组氨酸二醇磷酸酶类成员的基因；IV型菌毛功能所需的一组20个基因；以及一些编码抗毒素和/或免疫蛋白的条件必需基因。我们期望NeMeSys 2.0将促进人类主要细菌病原体的表型分析。脑膜炎奈瑟菌会导致危及生命的脑膜炎和败血症。在这里，Muir等人构建了该生物蛋白编码基因中已定义突变体的完整集合，他们利用这些突变体来鉴定其基本基因组，并阐明多个基因的功能。
The bacterium Neisseria meningitidis causes life-threatening meningitis and sepsis. Here, we construct a complete collection of defined mutants in protein-coding genes of this organism, identifying all genes that are essential under laboratory conditions. The collection, named NeMeSys 2.0, consists of individual mutants in 1584 non-essential genes. We identify 391 essential genes, which are associated with basic functions such as expression and preservation of genome information, cell membrane structure and function, and metabolism. We use this collection to shed light on the functions of diverse genes, including a gene encoding a member of a previously unrecognised class of histidinol-phosphatases; a set of 20 genes required for type IV pili function; and several conditionally essential genes encoding antitoxins and/or immunity proteins. We expect that NeMeSys 2.0 will facilitate the phenotypic profiling of a major human bacterial pathogen. The bacterium Neisseria meningitidis causes life-threatening meningitis and sepsis. Here, Muir et al. construct a complete collection of defined mutants in protein-coding genes of this organism, which they use to identify its essential genome and to shed light on the functions of multiple genes.
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影响因子：
17.3
作者：
Galperin, Michael Y.;Koonin, Eugene V.
通讯作者：
Koonin, Eugene V.
影响因子：
12.3
作者：
Baart GJ;Zomer B;de Haan A;van der Pol LA;Beuvery EC;Tramper J;Martens DE
通讯作者：
Martens DE
影响因子：
9.9
作者：
通讯作者：
--
影响因子：
9.9
作者：
de Berardinis, Veronique;Vallenet, David;Castelli, Vanina;Besnard, Marielle;Pinet, Agnes;Cruaud, Corinne;Samair, Sumitta;Lechaplais, Christophe;Gyapay, Gabor;Richez, Celine;Durot, Maxime;Kreimeyer, Annett;Le Fevre, Francois;Schaechter, Vincent;Pezo, Valerie;Doering, Volker;Scarpelli, Claude;Medigue, Claudine;Cohen, Georges N.;Marliere, Philippe;Salanoubat, Marcel;Weissenbach, Jean
通讯作者：
Weissenbach, Jean
影响因子：
14.9
作者：
Haft DH;DiCuccio M;Badretdin A;Brover V;Chetvernin V;O'Neill K;Li W;Chitsaz F;Derbyshire MK;Gonzales NR;Gwadz M;Lu F;Marchler GH;Song JS;Thanki N;Yamashita RA;Zheng C;Thibaud-Nissen F;Geer LY;Marchler-Bauer A;Pruitt KD
通讯作者：
Pruitt KD