Systematic discovery of antiphage defense systems in the microbial pangenome.
Systematic discovery of antiphage defense systems in the microbial pangenome.
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DOI：
10.1126/science.aar4120
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发表时间：
2018-03-02
期刊：
影响因子：
--
通讯作者：
Sorek R
中科院分区：
文献类型：
--
作者：
Doron S;Melamed S;Ofir G;Leavitt A;Lopatina A;Keren M;Amitai G;Sorek R
细菌和细菌之间的军备竞赛导致了复杂的抗噬菌体防御系统的发展，包括CRISPR-Cas和限制修饰系统。有证据表明，未知的防御系统位于微生物基因组中的“防御岛”中。我们全面的特点是细菌的防御武器库，通过检查基因家族聚集在原核基因组中的已知防御基因旁边。对候选防御系统进行系统工程改造，并在模型细菌中验证其抗噬菌体活性。我们报告了九个以前未知的抗噬菌体和一个抗质粒系统，这些系统广泛存在于微生物中，并强烈抵御外来入侵者。这些系统包括采用细菌鞭毛和凝聚素复合物的成分的系统。我们的数据还表明，动物、植物和细菌之间共享先天免疫成分的共同、古老祖先。
The arms race between bacteria and phages led to the development of sophisticated anti-phage defense systems, including CRISPR-Cas and restriction-modification systems. Evidence suggests that unknown defense systems are located in “defense islands” in microbial genomes. We comprehensively characterized the bacterial defensive arsenal by examining gene families that are clustered next to known defense genes in prokaryotic genomes. Candidate defense systems were systematically engineered and validated in model bacteria for their anti-phage activities. We report nine previously unknown anti-phage and one anti-plasmid systems that are widespread in microbes and strongly protect against foreign invaders. These include systems that adopted components of the bacterial flagella and condensin complexes. Our data also suggest a common, ancient ancestry of innate immunity components shared between animals, plants and bacteria.
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DOI：
10.1126/science.aad9822
发表时间：
2016-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dar D;Shamir M;Mellin JR;Koutero M;Stern-Ginossar N;Cossart P;Sorek R
通讯作者：
Sorek R
影响因子：
64.5
作者：
Caplan, Jeffrey L.;Mamillapalli, Padmavathi;Dinesh-Kumar, S. P.
通讯作者：
Dinesh-Kumar, S. P.
影响因子：
30.3
作者：
Depardieu, Florence;Didier, Jean-Philippe;Bikard, David
通讯作者：
Bikard, David
影响因子：
16.2
作者：
Essuman K;Summers DW;Sasaki Y;Mao X;DiAntonio A;Milbrandt J
通讯作者：
Milbrandt J
影响因子：
9.8
作者：
Burch-Smith TM;Schiff M;Caplan JL;Tsao J;Czymmek K;Dinesh-Kumar SP
通讯作者：
Dinesh-Kumar SP