An increase of curdlan productivity by integration of carbon/nitrogen sources control and sequencing dual fed-batch fermentors operation
An increase of curdlan productivity by integration of carbon/nitrogen sources control and sequencing dual fed-batch fermentors operation
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通过整合碳/氮源控制和双补料分批发酵罐操作顺序提高凝乳胶生产率
DOI：
10.1134/s000368381401013x
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发表时间：
2014
影响因子：
0.8
通讯作者：
Lin, C. C.
中科院分区：
文献类型：
--
作者：
Ma, L. W.;Wu, J. R.;Zhang, L. M.;Lin, C. C.
可德胶由土壤杆菌ATCC 31749在与细胞生长无关的氮限制条件下产生。 在发酵罐I中，通过补加铵溶液以提供充足的氮源和控制pH，促进了细胞的生长。获得了29 g/L的高细胞密度。然后将发酵罐I中的培养液接种到测序发酵罐II中，这减轻了在可得然胶生产期间对溶解氧的高需求和抑制性代谢副产物的积累。发酵罐I促进细胞生长。在发酵罐II中立即开始生产可得然胶。通过投加高碳氮比营养液和NaOH溶液调节pH值，确定了一种可行的可得然胶生产工艺。产率为0.98 g/（L·h），转化率为57%（w），凝胶多糖得率为67 g/L。这种新方法可以按比例放大，以在工业水平上显著降低成本。
Curdlan is produced by Agrobacterium sp. ATCC 31749 under nitrogen-limited conditions not associated with cell growth. By feeding ammonium solution to supply abundant nitrogen source and controlling pH in Fermentor I, cell growth was accelerated. High cell density of 29 g/L was attained. The culture broth in Fermentor I was then inoculated into sequencing Fermentor II which alleviated the high requirement for dissolved oxygen and accumulation of inhibitory metabolic by-products during curdlan production. Fermentor I promoted cell growth. Curdlan production started instantaneously in Fermentor II. By feeding nutrient solution with high carbon/nitrogen ratio and NaOH solution for pH adjustment, a feasible and optimal curdlan production process was formulated. The productivity, conversion efficiency and curdlan yield were achieved of 0.98 g/(L h), 57% (w) and 67 g/L, respectively. Such novel process can be scaled up for significant cost reduction at the industrial level.
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影响因子：
2.5
作者：
Drobne, Damjana;Milani, Marziale;Tatti, Francesco
通讯作者：
Tatti, Francesco
影响因子：
2.3
作者：
Sebastian Schädler;Claus Burkhardt;Andreas Kappler
通讯作者：
Sebastian Schädler;Claus Burkhardt;Andreas Kappler
农杆菌响应中涉及的基因转录和蛋白质表达的变化。
影响因子：
5
作者：
Chi-Chung Lin;Zhi-Yong Zheng;Li-Jun Yu;Xiao-Bei Zhan;Jian-Rong Wu
通讯作者：
Jian-Rong Wu
农杆菌中负责凝胶多糖生物合成的基因的序列和转录分析。
影响因子：
5
作者：
Wu, Jian-Rong;Jiang Yun;Zhang, Hong-Tao;Lin, Chi Chung;Yu, Xiao-Bing;Zheng, Zhi-Yong;Zhan, Xiaobei
通讯作者：
Zhan, Xiaobei
影响因子：
2.7
作者：
J. W. Lee;W. Yeomans;A. Allen;R. Gross;D. Kaplan
通讯作者：
J. W. Lee;W. Yeomans;A. Allen;R. Gross;D. Kaplan