Human JCV infections as a bio-anthropological marker of the formation of Brazilian Amazonian populations.
Human JCV infections as a bio-anthropological marker of the formation of Brazilian Amazonian populations.
复制标题
人类 JCV 感染作为巴西亚马逊种群形成的生物人类学标志。
DOI：
10.1371/journal.pone.0046523
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ishak R
中科院分区：
文献类型：
--
作者：
Cayres-Vallinoto IM;Vallinoto AC;Azevedo VN;Machado LF;Ishak Mde O;Ishak R
JC多瘤病毒(JC PolyomaVirus，JCV)是多核病毒科的成员。它对肾脏细胞呈趋向性，感染发生在不同种族背景的各种人群中。本研究调查了巴西亚马逊地区人群中JCV感染的流行情况，并描述了该病毒的分子和系统发育特征。对来自贝伦两个城市群体(健康受试者)、来自里约热内卢特罗姆贝塔斯地区的一名巴西非洲裔“quilombo”以及来自Wai-Wai、Uruu-Kaapor、Tembé、Assurini、Arara do Laranjal、Aukre、Parakanã、Surui和Munduruku村庄的土著印第安人的尿样进行了病毒检测，方法是利用聚合酶链式反应扩增VP1(230 Bp)和IG(610 Bp)区域。48个样本的核苷酸序列(440个核苷酸，NT)被提交给系统发育分析。结果证实，在健康受试者中出现了A型(Eu亚型)、B型(Af-2亚型，非洲人和我的亚型)和C型(Af-1亚型)；在非洲裔巴西人中出现了B型，Af-2和My亚型；在苏瑞印第安人中出现了B型，My亚型。一个意想不到的结果是在非洲人后裔中检测到另一种多瘤病毒--BKV。本研究首次表明，在巴西亚马逊地区出现了感染人类的JC和BK多瘤病毒。结果显示，该地区流行的菌株具有很大的遗传变异性，感染了一大群人。与这里调查的人口样本的种族起源有关的欧洲、亚洲和非洲亚型的存在，突显了JCV是研究人类早期迁徙的一个相当好的标志，反映了他们的早期和晚期历史。此外，对与病毒亚型相关的特定突变的鉴定表明，这些突变发生在病毒进入巴西亚马逊地区之后。
JC polyomavirus (JCV) is a member of the Polyomaviridae family. It presents a tropism to kidney cells, and the infection occurs in a variety of human population groups of different ethnic background. The present study investigated the prevalence of JCV infection among human populations from the Brazilian Amazon region, and describes the molecular and phylogenetic features of the virus. Urine samples from two urban groups of Belém (healthy subjects), one Brazilian Afro-descendant “quilombo” from the Rio Trombetas region, and native Indians from the Wai-Wai, Urubu-Kaapor, Tembé, Assurini, Arara do Laranjal, Aukre, Parakanã, Surui and Munduruku villages were investigated for the presence of the virus by amplifying VP1 (230 bp) and IG (610 bp) regions using a polymerase chain reaction. Nucleotide sequences (440 nucleotides, nt) from 48 samples were submitted to phylogenetic analysis. The results confirmed the occurrence of types A (subtype EU), B (subtypes Af-2, African and MY, Asiatic) and C (subtype Af-1) among healthy subjects; type B, subtypes Af-2 and MY, among the Afro-Brazilians; and type B, subtype MY, within the Surui Indians. An unexpected result was the detection of another polyomavirus, the BKV, among Afro-descendants. The present study shows, for the first time, the occurrence of JC and BK polyomaviruses infecting humans from the Brazilian Amazon region. The results show a large genetic variability of strains circulating in the region, infecting a large group of individuals. The presence of European, Asiatic and African subtypes associated to the ethnic origin of the population samples investigated herein, highlights the idea that JCV is a fairly good marker for studying the early migration of human populations, reflecting their early and late history. Furthermore, the identification of the specific mutations associated to the virus subtypes, suggests that these mutations have occurred after the entrance of the virus in the Amazon region of Brazil.
登录
查看更多内容
DOI：
10.1073/pnas.96.13.7484
发表时间：
1999-06-22
影响因子：
11.1
作者：
Laghi, L;Randolph, AE;Boland, CR
通讯作者：
Boland, CR
影响因子：
9.4
作者：
Agostini, HT;Ryschkewitsch, CF;Stoner, GL
通讯作者：
Stoner, GL
影响因子：
8.8
作者：
Azzi, A;Cesaro, S;Bosi, A
通讯作者：
Bosi, A
影响因子：
6.4
作者：
Engels, EA;Rollison, DE;Viscidi, RP
通讯作者：
Viscidi, RP
影响因子：
3.8
作者：
Agostini, HT;Deckhut, A;Stoner, GL
通讯作者：
Stoner, GL