Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells.
Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells.
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抗人胰腺导管腺癌细胞的大鼠单克隆抗体的产生。
DOI：
10.1089/mab.2016.0012
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发表时间：
2016
影响因子：
--
通讯作者：
Tachibana T
中科院分区：
文献类型：
--
作者：
Higashi K;Fujii N;Kushida M;Yamada K;Suzuki N;Saito K;Tachibana T
胰腺导管腺癌是一种侵袭性肿瘤，预后不良。在肿瘤可以根治性切除的情况下，早期发现肿瘤的生物标志物可能会改善预后。为了发现在原发胰腺癌中表达的新标记物，我们用大鼠髂骨内侧淋巴结法制备了一组针对胰腺导管腺癌细胞系BxPC3的单抗。1B5A5识别的抗原在细胞表面表达并分泌到BxPC3细胞的条件培养液中，鉴定为糖蛋白，分子量在60~90 kDa之间。在几种胰腺癌细胞系中观察到大范围的1B5A5抗原分子量。采用人类多器官肿瘤组织阵列的免疫组织化学显示，1B5A5抗原在胰腺癌、肺癌、胃癌、乳腺癌、膀胱癌、结肠癌和宫颈癌中的表达增强。免疫沉淀和蛋白质组学分析证实CEACAM6为1B5A5抗原。此外，免疫印迹分析结果表明，1B5A5表位位于CEACAM6的氨基末端区域。这些结果增加了我们的方法可能导致在相对较短的时间内发现癌症早期阶段的新生物标记物的可能性。
Pancreatic ductal adenocarcinoma is an aggressive tumor with a poor prognosis. Biomarkers that can detect the tumor in its early stages when it may be amenable to curative resection might improve prognosis. To discover novel markers expressed in primary pancreatic cancer, we generated a panel of monoclonal antibodies against pancreatic ductal adenocarcinoma cell line BxPC3 using a rat medial iliac lymph node method. The antigen recognized by 1B5A5 was expressed on the cell surface and secreted into the conditioned medium of BxPC3 cells, and characterized as glycoproteins with molecular mass between 60 and 90 kDa. A wide range of molecular weights of 1B5A5 antigen in several pancreatic cancer cell lines were observed. Immunohistochemistry using a human multiple organ tumor tissue array showed an enhanced expression of 1B5A5 antigen in pancreas, lung, stomach, breast, urinary bladder, colon, and cervix uteri cancers. Immunoprecipitation followed by proteomic analyses identified CEACAM6 as a 1B5A5 antigen. In addition, western blot analysis results indicated that the 1B5A5 epitope is located within an amino-terminal domain of CEACAM6. These results raised the possibility that our approach could lead to discovery of novel biomarkers for the early stage of cancers in a relatively short period of time.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
DOI：
10.1089/mab.2014.0075
发表时间：
2015-02-01
影响因子：
--
作者：
Higashi, Kiyoshi;Yagi, Masaki;Saito, Koichi
通讯作者：
Saito, Koichi
影响因子：
3.5
作者：
Lee, Ok-Jun;Son, Seung-Myoung;Hong, Kwon Pyo;Lee, Yong-Moon;Kim, Min-Young;Choi, Jae-Woon;Lee, Sang-Jeon;Song, Young-Jin;Kim, Hak Soon;Kim, Wun-Jae;Shin, See-Ok;Song, Hyung Geun
通讯作者：
Song, Hyung Geun
DOI：
10.20772/cancersci1985.78.1_54
发表时间：
1987
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
T. Iwamura;T. Katsuki;K. Ide
通讯作者：
T. Iwamura;T. Katsuki;K. Ide
具有不同胞质结构域的 CEACAM1 亚型在极化上皮细胞中表现出不同的定位、组织和粘附特性。
影响因子：
4
作者：
U. Sundberg;B. Obrink
通讯作者：
B. Obrink