FgMet3 and FgMet14 related to cysteine and methionine biosynthesis regulate vegetative growth, sexual reproduction, pathogenicity, and sensitivity to fungicides in Fusarium graminearum.
FgMet3 and FgMet14 related to cysteine and methionine biosynthesis regulate vegetative growth, sexual reproduction, pathogenicity, and sensitivity to fungicides in Fusarium graminearum.
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DOI：
10.3389/fpls.2022.1011709
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
禾谷镰刀菌（Fusarium graminearum）是一种广泛存在于小麦等谷类作物中的破坏性丝状真菌。半胱氨酸和甲硫氨酸是两种独特的含硫氨基酸，在蛋白质合成和细胞生命中起着重要的作用，但它们在F。graminearum仍然在很大程度上未知。我们在F. graminearum中，这是与半胱氨酸和蛋氨酸的合成。我们发现FgMet 3和FgMet 14定位于细胞质，并且它们之间存在相互作用。FgMet 3和FgMet 14缺失突变体（Δ FgMet 3和Δ FgMet 14）在营养生长、色素形成、性发育、穿透性和致病性等方面存在缺陷。外源添加半胱氨酸和蛋氨酸后，Δ FgMet 3和Δ FgMet 14的营养生长和穿透能力完全恢复，而Δ FgMet 3的有性生殖能力完全恢复，Δ FgMet 14的有性生殖能力部分恢复。Δ FgMet 3和Δ FgMet 14对刚果红胁迫的敏感性降低，对SDS、NaCl、KCl、山梨醇、甲萘醌和Zn离子胁迫的敏感性增加。此外，FgMet 3和FgMet 14非特异性地调节F.禾谷镰刀菌属真菌。FgMet3和FgMet14相互作用，共同调控了黑曲霉的生长发育、致病性、色素形成、对杀菌剂的敏感性和胁迫因子。禾谷早熟禾
Fusarium graminearum is a destructive filamentous fungus, which widely exists in wheat and other cereal crops. Cysteine and Methionine are unique sulfur-containing amino acids that play an essential role in protein synthesis and cell life, but their functions and regulation in F. graminearum remain largely unknown. Here we identified two proteins, FgMet3 and FgMet14 in F. graminearum, which are related to the synthesis of cysteine and methionine. We found FgMet3 and FgMet14 were localized to the cytoplasm and there was an interaction between them. FgMet3 or FgMet14 deletion mutants (ΔFgMet3 and ΔFgMet14) were deficient in vegetative growth, pigment formation, sexual development, penetrability and pathogenicity. With exogenous addition of cysteine and methionine, the vegetative growth and penetrability could be completely restored in ΔFgMet3 and ΔFgMet14, while sexual reproduction could be fully restored in ΔFgMet3 and partially restored in ΔFgMet14. ΔFgMet3 and ΔFgMet14 exhibited decreased sensitivity to Congo red stress and increased sensitivity to SDS, NaCl, KCl, Sorbitol, Menadione, and Zn ion stresses. Moreover, FgMet3 and FgMet14 nonspecifically regulate the sensitivity of F. graminearum to fungicides. In conclusion, FgMet3 and FgMet14 interacted to jointly regulate the development, pathogenicity, pigment formation, sensitivity to fungicides and stress factors in F. graminearum.
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影响因子：
5.6
作者：
Erayman M;Turktas M;Akdogan G;Gurkok T;Inal B;Ishakoglu E;Ilhan E;Unver T
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.5
作者：
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通讯作者：
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跨膜蛋白 FgSho1 通过 MAPK 模块 Ste50-Ste11-Ste7 调节禾谷镰刀菌中的真菌发育和致病性
影响因子：
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作者：
Gu, Qin;Chen, Yun;Ma, Zhonghua
通讯作者：
Ma, Zhonghua
DOI：
10.3390/jof8030295
发表时间：
2022-03-13
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Amich J
通讯作者：
Amich J