Effect of nitric oxide on catharanthine production and growth of Catharanthus roseus suspension cells.
Effect of nitric oxide on catharanthine production and growth of Catharanthus roseus suspension cells.
复制标题
一氧化氮对长春花碱产生和长春花悬浮细胞生长的影响。
DOI：
10.1002/bit.20334
复制
发表时间：
2005-02
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
以硝普钠（SNP）为一氧化氮（NO）供体，研究其对长春花悬浮细胞生长及长春质碱合成的影响。结果表明，高浓度（10.0和20.0 mmol/L）的SNP刺激C. roseus细胞，但抑制细胞的生长。低浓度的SNP（0.1和0.5mmol/L）促进C.玫瑰细胞，但对长春质碱的合成没有影响。在培养的第0天和第10天分别加入0.5和10.0 mmol/L的SNP，长春质碱的总产量达到最大，约为对照的3倍。NO诱导C.茉莉酸（JA）生物合成抑制剂布洛芬（IBU）和去甲二氢愈创木酸（NDGA）可显著抑制玫瑰细胞的增殖。结果提示，NO对长春质碱产生的刺激作用部分依赖于茉莉酸。
Sodium nitroprusside (SNP) was used as the donor of nitric oxide (NO) to investigate its effect on catharanthine synthesis and the growth of Catharanthus roseus suspension cells. The results showed that SNP at high concentrations (10.0 and 20.0 mmol/L) stimulated catharanthine formation of C. roseus cells, but inhibited growth of the cells. Low concentrations of SNP (0.1 and 0.5 mmol/L) enhanced the growth of C. roseus cells, but had no effect on catharanthine synthesis. The maximum total catharanthine production was achieved by the addition of 0.5 and 10.0 mmol/L SNP to the cultures at day 0 and day 10, respectively, being about threefold of the control. NO-induced catharanthine production of C. roseus cells was strongly suppressed by jasmonic acid (JA) biosynthesis inhibitor ibuprofen (IBU) and nordihydroguaiaretic (NDGA). The result suggests that the stimulatory role of NO on catharanthine production is partially JA-dependent.
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影响因子：
4.9
作者：
Gantet, P;Imbault, N;Doireau, P
通讯作者：
Doireau, P
DOI：
10.1046/j.1432-1327.2000.01564.x
发表时间：
2000-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Maccarrone, M;Van Zadelhoff, G;Finazzi-Agrò, A
通讯作者：
Finazzi-Agrò, A
影响因子：
--
作者：
Hu, XY;Neill, SJ;Tang, ZC
通讯作者：
Tang, ZC
影响因子：
3.8
作者：
Oikawa, A;Ishihara, A;Iwamura, H
通讯作者：
Iwamura, H
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F