Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation.
Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation.
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DOI：
10.1080/20002297.2021.1898838
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发表时间：
2021-03-12
影响因子：
4.5
通讯作者：
Giannecchini S
中科院分区：
文献类型：
--
作者：
Mamana AC;Stincarelli M;Sarmento DJS;Mendes Batista A;Tozetto-Mendoza TR;Gallottini M;De Abreu Pestana JOM;Braz-Silva PH;Giannecchini S
背景：多瘤病毒(PolyomaVirus，PYV)的microRNAs(MiRNAs)存在于多种生物体液中，可作为监测其持久性的相关病毒因子。目的：评价一种免疫抑制方案对PYV-miRNA-5p在口腔中状态的影响。材料和方法：采用实时荧光定量RT-PCR方法，对23例肾移植患者移植前后的唾液和血浆标本中的JCPyV、BKPyV、MCPyV miRNA-5p进行检测。结果：总体而言，在移植后的短时间内，患者表现出白细胞和淋巴细胞数量的减少以及肌酐水平的降低。在患者的临床治疗期间，大量唾液样本JCPyV和BKPyV miRNA-5p阳性(范围：26%-91%)，而配对血浆样本(范围：9%-35%)。在miRNA-5p阳性表达的两种多瘤病毒中，BKPyV在唾液中的阳性率最高(91%)，而MCPyV-miRNA-5p在唾液和血浆中均为阴性。与移植前相比，移植后唾液中JCPyV-miRNA-5p的表达显著降低。结论：综上所述，这些数据表明，在肾移植后不久应该对唾液中的多瘤病毒miRNA-5p进行进一步的研究，以评估其在早期病毒再激活中的潜在作用。
Background: MicroRNAs (miRNAs) of polyomavirus (PyV) are present in several biological fluids and are suggested to be relevant viral factors for monitoring its persistence. Aim: To evaluate the effect of an immunosuppressive regimen on the status of PyV-miRNA-5p in the oral cavity. Materials and Methods: The JCPyV, BKPyV, MCPyV miRNA-5p were investigated in paired saliva and plasma samples obtained from 23 patients before and shortly after renal-transplantation by using real-time RT-PCR. Results: Overall, within a short-time after transplantation, patients exhibited decreased numbers of leukocyte and lymphocyte as well as low levels of creatinine. During the clinical management of the patients, a significant amount of saliva samples were positive for JCPyV and BKPyV miRNA-5p (range: 26%-91%) compared to paired plasma samples (range: 9%-35%). Among the two polyomaviruses showing positive expression of miRNA-5p, BKPyV presented the highest positivity in saliva (91%) and MCPyV-miRNA-5p was constantly negative in both saliva and plasma samples. Compared to the time before transplantation, a significant reduction in the expression of JCPyV-miRNA-5p was observed in saliva samples obtained after transplantation. Conclusions: Altogether, these data suggest that additional investigations of polyomavirus miRNA-5p in saliva should be performed shortly after renal-transplantation to evaluate the potential role in early viral reactivation.
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DOI：
10.1007/s00428-007-0534-0
发表时间：
2008-04
期刊：
Virchows Archiv : an international journal of pathology
影响因子：
--
作者：
Kutsuna T;Zheng H;Abdel-Aziz HO;Murai Y;Tsuneyama K;Furuta I;Takano Y
通讯作者：
Takano Y
影响因子：
4.8
作者：
Lagatie O;Van Loy T;Tritsmans L;Stuyver LJ
通讯作者：
Stuyver LJ
影响因子：
8.8
作者：
Castroa, Talita;Domingues Fink, Maria Cristina;Gallottini, Marina
通讯作者：
Gallottini, Marina
影响因子：
2.7
作者：
Sarmento, Dmitry José de Santana;Palmieri, Michelle;Braz-Silva, Paulo Henrique
通讯作者：
Braz-Silva, Paulo Henrique
影响因子：
2.7
作者：
Calvignac-Spencer, Sebastien;Feltkamp, Mariet C. W.;Ehlers, Bernhard
通讯作者：
Ehlers, Bernhard