Immune cells in the normal ovary and spontaneous ovarian tumors in the laying hen (Gallus domesticus) model of human ovarian cancer.
Immune cells in the normal ovary and spontaneous ovarian tumors in the laying hen (Gallus domesticus) model of human ovarian cancer.
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DOI：
10.1371/journal.pone.0074147
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Luborsky JL
中科院分区：
文献类型：
--
作者：
Bradaric MJ;Penumatsa K;Barua A;Edassery SL;Yu Y;Abramowicz JS;Bahr JM;Luborsky JL
鸡的自发性卵巢癌在组织学和生物化学上与人类肿瘤相似。目的是确定鸡正常卵巢和卵巢肿瘤之间淋巴细胞含量是否存在差异，作为进一步研究的基础，以了解免疫在人类卵巢癌进展中的作用。使用灰阶和彩色多普勒超声选择母鸡，以确定它们是否具有正常或肿瘤形态。从卵巢（n = 6只母鸡）中分离细胞，并使用禽类CD 4和CD 8 T和B（Bu1a）细胞的抗体通过流式细胞术测定淋巴细胞数。 评估另一组母鸡（n =26）的卵巢切片，以验证肿瘤类型和分期，并通过形态计量学分析计数CD4、CD8和Bu1a免疫染色细胞。 流式细胞术和免疫组化显示卵巢肿瘤组织中T、B细胞数量明显多于正常卵巢组织。正常卵巢和卵巢肿瘤组织中CD4+细胞明显少于CD8+和Bu1a+细胞。与CD4+细胞相比，CD8+细胞是卵巢基质和卵泡中的主要T细胞亚型。Bu1a+细胞在正常卵巢和卵巢肿瘤的间质中被一致地发现，但与卵泡无关。免疫细胞的数量在晚期浆液性肿瘤中最高，与类浆液性和粘液性肿瘤相比。结果表明，与人类卵巢癌相似，鸡卵巢肿瘤中的免疫细胞比正常卵巢中的免疫细胞相对更多，并且在浆液性肿瘤中免疫细胞含量最高。因此，本研究为进一步研究卵巢癌自发模型中的肿瘤免疫应答奠定了基础，这将有助于研究免疫在早期卵巢癌进展中的作用以及在临床前疫苗试验中使用母鸡。
Spontaneous ovarian cancer in chickens resembles human tumors both histologically and biochemically. The goal was to determine if there are differences in lymphocyte content between normal ovaries and ovarian tumors in chickens as a basis for further studies to understand the role of immunity in human ovarian cancer progression. Hens were selected using grey scale and color Doppler ultrasound to determine if they had normal or tumor morphology. Cells were isolated from ovaries (n = 6 hens) and lymphocyte numbers were determined by flow cytometry using antibodies to avian CD4 and CD8 T and B (Bu1a) cells. Ovarian sections from another set of hens (n = 26) were assessed to verify tumor type and stage and to count CD4, CD8 and Bu1a immunostained cells by morphometric analysis. T and B cells were more numerous in ovarian tumors than in normal ovaries by flow cytometry and immunohistochemistry. There were less CD4+ cells than CD8+ and Bu1a+ cells in normal ovaries or ovarian tumors. CD8+ cells were the dominant T cell sub-type in both ovarian stroma and in ovarian follicles compared to CD4+ cells. Bu1a+ cells were consistently found in the stroma of normal ovaries and ovarian tumors but were not associated with follicles. The number of immune cells was highest in late stage serous tumors compared to endometrioid and mucinous tumors. The results suggest that similar to human ovarian cancer there are comparatively more immune cells in chicken ovarian tumors than in normal ovaries, and the highest immune cell content occurs in serous tumors. Thus, this study establishes a foundation for further study of tumor immune responses in a spontaneous model of ovarian cancer which will facilitate studies of the role of immunity in early ovarian cancer progression and use of the hen in pre-clinical vaccine trials.
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影响因子：
4.7
作者：
Ansenberger, Kristine;Zhuge, Yan;Lagman, Jo Ann J.;Richards, Cassandra;Barua, Animesh;Bahr, Janice M.;Hales, Dale Buchanan
通讯作者：
Hales, Dale Buchanan
影响因子：
82.9
作者：
Dinulescu, DM;Ince, TA;Jacks, T
通讯作者：
Jacks, T
DOI：
10.1530/jrf.0.1140011
发表时间：
1998-09-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Barua, A;Yoshimura, Y;Tamura, T
通讯作者：
Tamura, T
影响因子：
2
作者：
Davison, TF
通讯作者：
Davison, TF
影响因子：
2.3
作者：
Barua, Animesh;Abramowicz, Jacques S.;Luborsky, Judith L.
通讯作者：
Luborsky, Judith L.