Rat psychomotor vigilance task with fast response times using a conditioned lick behavior.
Rat psychomotor vigilance task with fast response times using a conditioned lick behavior.
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DOI：
10.1016/j.bbr.2010.07.041
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Rector, David M.
中科院分区：
文献类型：
--
作者：
Walker, Jennifer L.;Walker, Brendan M.;Fuentes, Fernanda Monjaraz;Rector, David M.
关键词：
对睡眠控制的生理机制的研究需要具有快速反应时间（<300ms）的动物心理警觉任务（PVT）。大鼠提供了一个很好的PVT模型，因为胡须刺激产生快速和强大的皮层诱发反应，动物可以被训练舔刺激后。我们之前的实验使用剥夺为基础的方法，以最大限度地提高动机的操作性条件反射。然而，剥夺会影响生理和神经行为效应。为了在不缺水的情况下保持动力，我们对大鼠进行了固定和头部约束，然后训练它们舔10%的蔗糖溶液以响应胡须刺激。在大约8次训练后，动物对刺激产生了大于80%的正确命中。在训练的过程中，反应时间变得更快，正确的命中率提高了。在通过温和处理实现的3、6和12小时睡眠剥夺后，检查PVT中的性能。在6小时和12小时的睡眠剥夺后，正确命中率显着下降，而在12小时的睡眠剥夺后，反应时间显着增加。虽然在行为上动物似乎是清醒的，但我们观察到在错过之前EEG δ功率显著增加。具有快速反应时间的大鼠PVT允许研究睡眠剥夺效应、任务时间和药理学试剂。快速的响应时间也允许更接近正在进行的人类协议的平行研究。
Investigations into the physiological mechanisms of sleep control require an animal psychomotor vigilance task (PVT) with fast response times (<300ms). Rats provide a good PVT model since whisker stimulation produces a rapid and robust cortical evoked response, and animals can be trained to lick following stimulation. Our prior experiments used deprivation-based approaches to maximize motivation for operant conditioned responses. However, deprivation can influence physiological and neurobehavioral effects. In order to maintain motivation without water deprivation, we conditioned rats for immobilization and head restraint, then trained them to lick for a 10% sucrose solution in response to whisker stimulation. After approximately 8 training sessions, animals produced greater than 80% correct hits to the stimulus. Over the course of training, reaction times became faster and correct hits increased. Performance in the PVT was examined after 3, 6 and 12 hours of sleep deprivation achieved by gentle handling. A significant decrease in percent correct hits occurred following 6 and 12 hours of sleep deprivation and reaction times increased significantly following 12 hours of sleep deprivation. While behaviorally the animals appeared to be awake, we observed significant increases in EEG delta power prior to misses. The rat PVT with fast response times allows investigation of sleep deprivation effects, time on task and pharmacological agents. Fast response times also allow closer parallel studies to ongoing human protocols.
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