Stress-activated afferent inputs into the anterior parvicellular part of the paraventricular nucleus of the hypothalamus: Insights into urocortin 3 neuron activation.
Stress-activated afferent inputs into the anterior parvicellular part of the paraventricular nucleus of the hypothalamus: Insights into urocortin 3 neuron activation.
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DOI：
10.1016/j.brainres.2015.03.009
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发表时间：
2015-06-22
期刊：
影响因子：
2.9
通讯作者：
Li, Chien
中科院分区：
文献类型：
--
作者：
van-Hover, Christine;Li, Chien
尿皮质素3（Urocortin 3，UCN 3）是促肾上腺皮质激素释放因子（corticotropin-releasing factor，CRF）家族的成员，在协调应激反应中起重要作用。下丘脑室旁核（PVHap）的前小细胞部分中的Ucn 3神经元提供到下丘脑腹内侧核（VMH）的显著输入，VMH是众所周知的饱腹感中心，其中Ucn 3起抑制进食和调节血糖水平的作用。在本研究中，我们首先确定了Ucn 3在PVHap的表达是由急性束缚应激刺激。然后，我们进行了逆行追踪与荧光金（FG）结合免疫组化Fos作为神经元激活的标记物后，约束应力，以确定压力激活传入输入到PVHap。在终纹床核、外侧隔核、杏仁内侧核和下丘脑的VMH、弓状核、后核和乳头体腹前核等核团中发现了大量FG/Fos双标细胞。在脑干，FG/Fos阳性细胞见于中脑导水管周围灰质、孤束核和延髓腹外侧区。总之，本研究表明，急性应激迅速刺激PVHap中的Ucn 3表达，并确定了投射到PVHap的特定应激敏感脑区。这些区域在介导PVHap中的Ucn 3神经元的应激诱导的激活中具有潜在的重要性。
Urocortin 3 (Ucn 3) is a member of the corticotropin-releasing factor family, which plays a major role in coordinating stress responses. Ucn 3 neurons in the anterior parvicellular part of the paraventricular nucleus of the hypothalamus (PVHap) provide prominent input into the ventromedial nucleus of the hypothalamus (VMH), a well known satiety center, where Ucn 3 acts to suppress feeding and modulate blood glucose levels. In the present study, we first determined that Ucn 3 expression in the PVHap was stimulated by acute restraint stress. We then performed retrograde tracing with fluorogold (FG) combined with immunohistochemistry for Fos as a marker for neuronal activation after restraint stress to determine the stress-activated afferent inputs into the PVHap. Substantial numbers of FG/Fos double labeled cells were found in the bed nucleus of the stria terminalis, lateral septal nucleus, the medial amygdala, and a number of nuclei in the hypothalamus including the VMH, the arcuate nucleus, the posterior nucleus, and the ventral premammillary nucleus. In the brainstem, FG/Fos positive cells were found in the periaqueductal gray, the nucleus of the solitary tract, and the ventrolateral medulla. In conclusion, the present study showed that acute stress rapidly stimulates Ucn 3 expression in the PVHap and identified specific stress-sensitive brain areas that project to the PVHap. These areas are potentially important in mediating the stress-induced activation of Ucn 3 neurons in the PVHap.
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影响因子：
--
作者：
EFERAKEYA, A;BUNAG, RD
通讯作者：
BUNAG, RD
DOI：
10.1111/j.1749-6632.1997.tb48289.x
发表时间：
1997-01-01
期刊：
PSYCHOBIOLOGY OF POSTTRAUMATIC STRESS DISORDER
影响因子：
--
作者：
Davis, M;Walker, DL;Lee, YL
通讯作者：
Lee, YL
影响因子：
2.9
作者：
Cavalcante, Judney C.;Bittencourt, Jackson C.;Elias, Carol F.
通讯作者：
Elias, Carol F.
影响因子：
2.9
作者：
Gaykema, Ronald P. A.;Chen, Chiao-Chi;Goehler, Lisa E.
通讯作者：
Goehler, Lisa E.
影响因子：
3.3
作者：
Angeles-Castellanos, M.;Mendoza, J.;Escobar, C.
通讯作者：
Escobar, C.