Trace-Element Incorporation into Intracellular Pools Uncovers Calcium-Pathways in a Coccolithophore.

Trace-Element Incorporation into Intracellular Pools Uncovers Calcium-Pathways in a Coccolithophore.
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DOI:
10.1002/advs.201700088
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发表时间:
2017-10
期刊:
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子:
--
通讯作者:
Scheffel A
Scheffel A
中科院分区:
其他
文献类型:
--
作者:
Gal A;Sviben S;Wirth R;Schreiber A;Lassalle-Kaiser B;Faivre D;Scheffel A

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许多生物体在严格的生物控制下从结晶的前体阶段形成矿物。这些前体相的形成、运输和沉积的动态细胞内过程具有挑战性。最近发现了一种不寻常的情况,即钙化的埃米利亚·赫胥黎(Emiliania huxleyi),因为细胞含有一个充满浓缩的Ca和P相的隔室,但最终的方解石晶体在不同的隔室中成核,是不含P的。因此,富钙磷矿池与矿化过程的联系仍不清楚。在这里,使用Sr的脉冲追踪实验来标记E中的富Ca-P相。huxleyi细胞,以及低温X射线吸收光谱和分析型透射电子显微镜,以跟踪细胞内的Sr。发现Sr首先在富Ca-P相中发现,然后被并入方解石中。这表明细胞用于构建方解石的钙来自富含Ca-P的池。
Many organisms form minerals from precursor phases that crystallize under strict biological control. The dynamic intracellular processes of formation, transport, and deposition of these precursor phases are challenging to identify. An unusual situation is recently revealed for the calcifying alga Emiliania huxleyi, as the cells contain a compartment filled with a concentrated Ca and P phase but the final calcite crystals, which are nucleated in a different compartment, are P‐free. Thus, the connection of the Ca–P‐rich pool to the mineralization process remains unclear. Here, pulse‐chase experiments are used with Sr to label the Ca–P‐rich phase in E. huxleyi cells, and cryo X‐ray absorption spectroscopy and analytical transmission electron microscopy to follow the Sr within cells. It is found that Sr is first found in the Ca–P‐rich phase and then becomes incorporated into the calcite. This demonstrates that the calcium used by the cells to build calcite originates from the Ca–P‐rich pool.
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