Phylogenetic and pathotypical analysis of two virulent Newcastle disease viruses isolated from domestic ducks in China.
Phylogenetic and pathotypical analysis of two virulent Newcastle disease viruses isolated from domestic ducks in China.
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我国鸭源两种强效新城疫病毒的系统发育及致病型分析
DOI：
10.1371/journal.pone.0025000
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Zhang S;Wang X;Zhao C;Liu D;Hu Y;Zhao J;Zhang G
对从中国鸭暴发疫情中分离到的两株新城疫强毒的特性进行了研究。系统发育分析表明，这两株病毒都与II类病毒聚在一起，其中一个与基因vii新城疫病毒亲缘关系较近，另一个与基因ix亲缘关系较近。融合(F)蛋白裂解位点的氨基酸序列分析表明，两个分离株在裂解位点均含有毒力基序112RRQK/RRF117。这两种新城疫病毒对完全敏感的鸡具有严重的致病性，导致100%的死亡。其中一株分离株对家鸭也有一定的致病性。本研究提示，新城疫病毒在中国地区的家鸭中流行不止一种基因型，病毒可能在鸡和鸭之间传播。
Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to genotype IX. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that both isolates contained the virulent motif 112RRQK/RRF117 at the cleavage site. The two NDVs had severe pathogenicity in fully susceptible chickens, resulting in 100% mortality. One of the isolates also demonstrated some pathogenicity in domestic ducks. The present study suggests that more than one genotype of NDV circulates in domestic ducks in China and viral transmission may occur among chickens and domestic ducks.
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