Accordion-like honeycombs for tissue engineering of cardiac anisotropy.
Accordion-like honeycombs for tissue engineering of cardiac anisotropy.
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DOI：
10.1038/nmat2316
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发表时间：
2008-12
期刊：
影响因子：
41.2
通讯作者：
Freed, Lisa E.
中科院分区：
文献类型：
--
作者：
Engelmayr, George C., Jr.;Cheng, Mingyu;Bettinger, Christopher J.;Borenstein, Jeffrey T.;Langer, Robert;Freed, Lisa E.
组织工程移植物可能在心肌修复中有用，然而先前的支架在结构上与再现心脏各向异性不相容。利用微加工技术，一种新的手风琴状蜂窝状微结构呈现在聚（甘油癸二酸酯），以产生多孔，弹性体的3-D支架具有可控的刚度和各向异性。带有培养的新生大鼠心脏细胞的手风琴状蜂窝支架通过以下方式证明了其实用性：（1）与天然成年大鼠右心室心肌相比，机械性能紧密匹配，刚度由聚合物固化时间控制;（2）通过电场刺激诱导心脏细胞收缩性方向依赖性电激发阈值（p<0.05）;和（3）比各向同性对照支架更大的心脏细胞排列（p<0.0001）。原型双层支架与3-D互连孔网络产生电兴奋的移植物与多层新生大鼠心脏细胞。因此，手风琴样蜂窝可以克服以前支架的主要结构-机械限制，促进具有对齐的心脏细胞和更接近天然心肌的机械性质的移植物的形成。
Tissue engineered grafts may be useful in myocardial repair, however previous scaffolds have been structurally incompatible with recapitulating cardiac anisotropy. Utilizing microfabrication techniques, a novel accordion-like honeycomb microstructure was rendered in poly(glycerol sebacate) to yield porous, elastomeric 3-D scaffolds with controllable stiffness and anisotropy. Accordion-like honeycomb scaffolds with cultured neonatal rat heart cells demonstrated utility via: (1) closely matched mechanical properties compared to native adult rat right ventricular myocardium, with stiffnesses controlled by polymer curing time; (2) heart cell contractility inducible by electric field stimulation with directionally-dependent electrical excitation thresholds (p<0.05); and (3) greater heart cell alignment (p<0.0001) than isotropic control scaffolds. Prototype bilaminar scaffolds with 3-D interconnected pore networks yielded electrically excitable grafts with multi-layered neonatal rat heart cells. Accordion-like honeycombs can thus overcome principal structural-mechanical limitations of previous scaffolds, promoting the formation of grafts with aligned heart cells and mechanical properties more closely resembling native myocardium.
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影响因子：
4.9
作者：
Crapo, Peter M.;Gao, Jin;Wang, Yadong
通讯作者：
Wang, Yadong
DOI：
10.1007/s10047-003-0230-z
发表时间：
2003-01-01
期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
McCulloch, Andrew D.
影响因子：
14
作者：
Chen, Qi-Zhi;Bismarck, Alexander;Boccaccini, Aldo R.
通讯作者：
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