Correlated electrical and optical analysis of single nanoparticles and biomolecules on a nanopore-gated optofluidic chip.
Correlated electrical and optical analysis of single nanoparticles and biomolecules on a nanopore-gated optofluidic chip.
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DOI：
10.1021/nl502400x
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发表时间：
2014-08-13
期刊：
影响因子：
10.8
通讯作者：
Schmidt H
中科院分区：
文献类型：
--
作者：
Liu S;Zhao Y;Parks JW;Deamer DW;Hawkins AR;Schmidt H
对单个生物纳米颗粒的分析大大提高了我们对基本生物过程的理解，但也迅速成为个性化医疗新兴领域的分子诊断应用相关。已经开发了用于检测和分析单个生物分子的光学和电学方法，但是它们通常不一致地和以合适的集成形式使用以允许具有高通量的多模式分析。在这里，我们报告了一个双模式的电气和光学单纳米粒子传感设备的能力，将无法单独与每种技术。该新方法基于具有集成纳米孔的光流体芯片，该集成纳米孔用作智能门，以控制单个纳米颗粒向光学激发区域的递送，从而快速连续地进行无集成光学分析。我们演示了荧光纳米珠的电光流体尺寸区分、单个荧光标记流感病毒的电光检测，以及以高达100%的保真度识别相同尺寸荧光纳米颗粒混合物中的单个病毒。
The analysis of individual biological nanoparticles has significantly advanced our understanding of fundamental biological processes but is also rapidly becoming relevant for molecular diagnostic applications in the emerging field of personalized medicine. Both optical and electrical methods for the detection and analysis of single biomolecules have been developed, but they are generally not used in concert and in suitably integrated form to allow for multimodal analysis with high throughput. Here we report on a dual-mode electrical and optical single-nanoparticle sensing device with capabilities that would not be available with each technique individually. The new method is based on an optofluidic chip with an integrated nanopore that serves as a smart gate to control the delivery of individual nanoparticles to an optical excitation region for ensemble-free optical analysis in rapid succession. We demonstrate electro-optofluidic size discrimination of fluorescent nanobeads, electro-optical detection of single fluorescently labeled influenza viruses, and the identification of single viruses within a mixture of equally sized fluorescent nanoparticles with up to 100% fidelity.
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影响因子：
3.6
作者：
Yin, Dongliang;Deamer, David W.;Hawkins, Aaron R.
通讯作者：
Hawkins, Aaron R.
影响因子：
48
作者：
Fan, Xudong;Yun, Seok-Hyun
通讯作者：
Yun, Seok-Hyun
DOI：
10.1073/pnas.1103554108
发表时间：
2011-05-31
影响因子：
11.1
作者：
Fields, Alexander P.;Cohen, Adam E.
通讯作者：
Cohen, Adam E.
影响因子：
19.6
作者：
Keyser, Ulrich F.;Koeleman, Bernard N.;Dekker, Cees
通讯作者：
Dekker, Cees
影响因子：
38.3
作者：
Wei, Ruoshan;Gatterdam, Volker;Rant, Ulrich
通讯作者：
Rant, Ulrich